肿瘤的侵袭、转移不仅取决于肿瘤细胞的遗传和表观遗传特性，同样也有赖于微环境中巨噬细胞极化等基质细胞表型变化的影响。作为细胞间信号通讯的方式，外泌体miRNA介导肿瘤细胞和基质细胞的信息沟通，在肿瘤微环境的重塑过程中发挥了重要作用。然而，以外泌体miRNA为中介，探索肿瘤细胞诱导巨噬细胞极化的研究甚少。结合课题组前期验证乳腺癌细胞诱导巨噬细胞M2极化的结果，本课题以组蛋白修饰为切入点，探讨乳腺癌细胞外泌体miRNA诱导巨噬细胞M2极化重塑肿瘤微环境的分子机制。课题既结合肿瘤生物学的机制研究，同时将转化医学的理念贯穿于课题实施中，不仅有助于对外泌体miRNA作为信号通讯载体沟通肿瘤微环境细胞间交流意义的深入理解，也为该信息转导机制作为临床诊治的创新靶点提供理论支持。

The invasion and metastasis of tumor depends not only on the genetic and epigenetic properties of tumor cells, but also on the phenotypic changes of stromal cells such as macrophage polarization in the microenvironment. As a means of cell-to-cell communication, exosome miRNA mediates the communication between tumor cells and stromal cells and plays an important role in the remodeling of the tumor microenvironment. However, little research has been done to explore the macrophage polarization induced by cancer cell-derived miRNA. Combined with the results of previous studies of verifying macrophage M2 polarization induced by breast cancer cells, this study will focus on histone modification as an entry point to investigate the macrophage M2 polarization induced by breast cancer cell-derived exosome miRNA. The subject integrates the mechanism study of tumor biology, and emphasizes the concept of translational medicine, which will not only helps to understand the significance of exosome miRNA as the signal communication carrier between the cells of the tumor microenvironment, but also provides a theoretical support for the information transduction mechanism as an innovative target for clinical diagnosis and treatment.