影响光动力治疗（PDT）效果的瓶颈在于如何根据病人的个体差异精准化剂量，甚至通过在体测评进行实时的剂量调整和优化。本申请利用光学空间频率域成像（SFDI）的优势，针对SFDI应用于鲜红斑痣血管靶向PDT在体测评时存在的科学问题展开研究：（1）研究适合鲜红斑痣血管靶向PDT测评的宽场非接触成像系统，研究基于SFDI的在线表面形貌测量和光强矫正方法；（2）针对光剂量在体测评，研究结合调制成像的、具有深度分辨能力的层状组织光学参数定量反演方法；研究大测量量下的光学参数快速反演方法；（3）针对光敏剂剂量在体测评，通过分离组织本征光学参数对荧光的耦合，研究由表观测量定量反演光敏剂浓度分布的方法；研究空间频率在改善荧光目标重建对比度中的作用；（4）研究个性化、精准化治疗光剂量的硬件实现方法，以及鲜红斑痣血管靶向PDT在体测评的临床应用方案。本研究也将对其它疾病的PDT在体测评、光敏剂研制具有推进作用。

The key problem influcing the curative effect of Photodynimac therapy(PDT)is howe to decide the precise dosimetry for individual patient and even provide the real-time adjustment of dose. In this proposal, taking advantage of the optical spatial frequency domain imaging (SFDI),the problems associting with SFDI technique in assessment of vascular-targeted PDT for port wine stains are dealt with. The main research content include: (1)investigate the SFDI measurment system which is not only suitable for assessment the vascular-targeted PDT of port wine stains but also able to provide on-line morphology measurement of the target tissue for rectifying the light intensity; (2) for the in vivo clinical assessment of light dosage, we will investiage the method, which is a hybrid of modulated imaging and a qiantitative method for depth-resolving the optical properties of layerd turbid tissue ,and the fast reconstruction method with the dense sampling datasets for quatitative obtaining the intrinsic optical parameters of layered inhomogenous tissue; (3)for the in vivo clinica assesement of photosensitizer dose,through the decoupling of fluorescence and intrinsic opticla parameters of tissue, we will investigate the quantitative method for reconstruction photosensitizer concentration from the measurement;(4) We will investigate the hardware for realizing the individual and precise delivery of light dose. In addition, the scheme for clinical application of the in vivo assesement of vascular-targeted PDT of port wine stains will been investigated. The reasearch will also provide reference to the in vivo PDT assessment of other illness and the development of the photosensitizer.