非甾体类消炎止痛药物（Nonsteroidal Anti-inflammatory Drugs，NSAIDs）是临床广泛使用的药物之一，同时也是环境中被频繁检出的一类新型环境污染物。NSAIDs污染暴露对非靶生物的潜在影响是非常值得关注的生态学问题之一。研究表明NSAIDs对生物产生毒性的过程和氧化应激胁迫密切相关，其中Nrf2(NF-E2-related factor2)是生物体内应对氧化应激反应的重要转录因子。本项目以枝角类浮游动物大型溞为对象，研究NSAIDs对大型溞的毒性效应及氧化应激胁迫，阐明转录因子Nrf2在大型溞应对NSAIDs暴露中的作用及其保护机制；揭示Nrf2响应变化与NSAIDs暴露对大型溞毒性及其种群变化影响之间关系；探讨NSAIDs暴露对浮游动物生长、发育和繁殖及种群变化的影响，为NSAIDs的生态风险评价提供科学依据。

Nonsteroidal Anti-inflammatory Drugs (NSAIDs) are among widely used pharmaceuticals, which were detected frequently in natural waterborne as emerging contaminants. It is worth of studying of the potential adverse impact derived from the NSAIDs exposure to non-targeted organisms. It was reported that the toxic effects of NSAIDs are involved in oxidative stress, in which Nrf2(NF-E2-related factor2) plays a pivotal role in regulation of antioxidant genes in organisms. In the present project, it focus on the study of the oxidative stress responses of Daphnia magna in face of the exposure of NSAIDs in water, investigation of the role and its regulation mechanism of transcriptional factor Nrf2 in the antioxidant processes of Daphnia magna, and analysis of the relationship between responses of Nrf2 at gene and protein levels and toxic effects, the changes of population structure of Daphnia magna under the exposure of NSAIDs, elucidation of the impact of NSAIDs on the growth, development, reproduction, and even the population structure change of Daphnia magna. The project will provide more insights into the toxic mechanisms of NSAIDs and also basic information for its ecological risks assessment of NSAIDs in aquatic environments.