近年来涌现出来的基因编码的蛋白质光敏剂，使得光动力作用或单线态氧发生的亚细胞定位，变得精细可控。因而蛋白质光敏剂的光动力作用，有望实现对特定亚细胞部位或特定功能蛋白的靶向氧化性调控。项目拟将蛋白质光敏剂毒杀红KillerRed、迷你单miniSOG、单蛋敏SOPP在AR4-2J、CHO-K1、GH3细胞亚细胞定位表达，研究细胞质膜、线粒体、溶酶体、内质网定位的光动力作用，对CCK1、P2Y1、H1、TRH受体本身，及对其所介导的胞浆钙振荡的调控作用；研究光动力作用对受体有效性的调控；研究单线态氧和超氧阴离子顺序氧化对受体活性的影响。改变选择性光照光斑的大小、光强，阐明细胞局部对整体的影响，及各个细胞对整个细胞团块的影响。通过这些研究有望将蛋白质光敏剂的光动力作用，转变为有效的细胞生理学研究工具。

The emergence in recent years of genetically-encoded protein photosensitisers has made it possible to target precisely photodynamic action or singlet oxygen to subcellular organelles or functional proteins. Therefore it has become possible to oxidatively regulate speciffic subcellualr organnelles and functional proteins. We aim to express protein photosensitisers KillerRed, miniSOG, SOPP at the plasma membrane, mitochondria, lysosomes, or the endoplasmic reticulum in AR4-2J, CHO-K1, and GH3 cells, to compare the photodynamic modulation of CCK1, P2Y1, H1, TRH1 receptors and the modulation of the receptor-mediated cytosolic calcium oscillations. We also aim to examine photodynamic regulation of receptor efficacy, and to examine the sequential regulation of receptor by singlet oxygen and superoxide anion. Selective illumination techniques have now made it possible to quantitate the influence of cellular region on the whole cell, and selected cells on the whole cell cluster. Such works will help to establish protein photosensitiser photodynamic action as powerful tools in cell physiology research.