Photodynamic Activation of Cholecystokinin 1 Receptor with Different Genetically Encoded Protein Photosensitizers and from Varied Subcellular Sites.
Photodynamic Activation of Cholecystokinin 1 Receptor with Different Genetically Encoded Protein Photosensitizers and from Varied Subcellular Sites.
复制标题
使用不同基因编码的蛋白质光敏剂和来自不同亚细胞位点的胆囊收缩素 1 受体的光动力激活
DOI：
10.3390/biom10101423
复制
发表时间：
2020-10-08
期刊：
影响因子：
5.5
通讯作者：
Cui ZJ
中科院分区：
文献类型：
--
作者：
Li Y;Cui ZJ
Cholecystokinin 1 receptor (CCK1R) is activated by singlet oxygen (1O2) generated in photodynamic action with sulphonated aluminum phthalocyanine (SALPC) or genetically encoded protein photosensitizer (GEPP) KillerRed or mini singlet oxygen generator (miniSOG). A large number of GEPP with varied 1O2 quantum yields have appeared recently; therefore, in the present work, the efficacy of different GEPP to photodynamically activate CCK1R was examined, as monitored by Fura-2 calcium imaging. KillerRed, miniSOG, miniSOG2, singlet oxygen protein photosensitizer (SOPP), flavin-binding fluorescent protein from Methylobacterium radiotolerans with point mutation C71G (Mr4511C71G), and flavin-binding fluorescent protein from Dinoroseobacter shibae (DsFbFP) were expressed at the plasma membrane (PM) in AR4-2J cells, which express endogenous CCK1R. Light irradiation (KillerRed: white light 85.3 mW‧cm−2, 4’ and all others: LED 450 nm, 85 mW·cm−2, 1.5′) of GEPPPM-expressing AR4-2J was found to all trigger persistent calcium oscillations, a hallmark of permanent photodynamic CCK1R activation; DsFbFP was the least effective, due to poor expression. miniSOG was targeted to PM, mitochondria (MT) or lysosomes (LS) in AR4-2J in parallel experiments; LED light irradiation was found to all induce persistent calcium oscillations. In miniSOGPM-AR4-2J cells, light emitting diode (LED) light irradiation-induced calcium oscillations were readily inhibited by CCK1R antagonist devazepide 2 nM; miniSOGMT-AR4-2J cells were less susceptible, but miniSOGLS-AR4-2J cells were not inhibited. In conclusion, different GEPPPM could all photodynamically activate CCK1R. Intracellular GEPP photodynamic action may prove particularly suited to study intracellular GPCR.
登录
查看更多内容
光动力生理学 - 使用蛋白质光敏剂进行细胞生理学中的光纳米操作
影响因子：
4
作者：
Jiang HN;Li Y;Cui ZJ
通讯作者：
Cui ZJ
影响因子：
4
作者：
Djeungoue-Petga, Marie-Ange;Hebert-Chatelain, Etienne
通讯作者：
Hebert-Chatelain, Etienne
影响因子：
5.5
作者：
Cui, ZJ;Kanno, T
通讯作者：
Kanno, T
DOI：
10.1111/tra.12619
发表时间：
2019-03
期刊：
Traffic (Copenhagen, Denmark)
影响因子：
--
作者：
Dores MR;Trejo J
通讯作者：
Trejo J
影响因子：
7.3
作者：
Bucinskaite, V;Kurosawa, M;Lundeberg, T
通讯作者：
Lundeberg, T