ω-羟基脂肪酸是合成生物可降解聚合材料的重要单体，由于其羟基位于不活泼的碳链端，传统的化学工艺很难特异性地合成此类化合物；废弃油脂资源丰富，利用微生物将其转化制备高附加值的ω-羟基脂肪酸已成为国际上的研究热点。针对生物转化过程中酶的产物专一性不高、转化过程效率低等问题，拟开展不同来源脂肪酸ω-羟化酶基因的克隆及功能评价、脂肪酸ω-羟化酶的改造、以废弃油脂为底物合成羟基脂肪酸合成体系等研究，解析脂肪酸ω-羟化酶催化特异性羟化的机制及酶结构和功能的关系，创制选择性的羟化酶，在此基础上，构建可以转化重要生物质资源糖和废弃油脂制备特殊羟基脂肪酸的工程菌，合成专一性的ω1、ω2及ω3等羟基脂肪酸，解析影响工程菌转化的限制因素，提升工程菌的转化效率，为生物法合成特定的羟基脂肪酸奠定基础。

ω-Hydroxy fatty acids are important precursors for biodegradable polymer materials. Due to the hydroxyl group in the inactive terminal of the carbon chain, it is difficult to specifically synthesize these compounds using traditional chemical processes. Waste oils are abundant resources. Therefore, microbial preparation of ω-hydroxy fatty acids using these resources is attractive. However the microbial process is facing problems with low enzymatic efficiency and poor product specificity, etc. In this study, fatty acid ω-hydroxylases of different origins will be cloned and evaluated. Then, site-directed mutagenesis strategies will be used to increase the enzyme activity, and product specificity, etc. Finally, a heterologous system will be constructed to convert waste oils efficiently to their ω-hydroxyl products using fermentation technology. This study is expected to lay the foundation for microbial synthesis of ω-hydroxy fatty acids.