拟南芥HDA15与HY5互作调控光信号转导的分子机制

光是调节植物生长发育最重要的环境信号因子之一。组蛋白去乙酰化酶广泛参与光调控的基因表达与发育过程，然而其作用机制尚不清楚。我们前期的研究表明，拟南芥RPD3/HDA1型组蛋白去乙酰化酶HDA15与光信号转导途径中重要的转录因子HY5发生互作，并共同抑制幼苗下胚轴伸长及相关基因的表达；此外，HDA15参与维持HY5蛋白在拟南芥幼苗中的稳定性。在此基础上，本项目拟进行以下研究：（1）分析HDA15和HY5在调控拟南芥下胚轴伸长途径中的遗传关系；（2）解析HDA15和HY5互作共同调控的靶基因网络及转录调控机制；（3）探索HDA15对HY5蛋白稳定性的调节机制。本研究将阐明HDA15-HY5互作调控光信号转导的分子机理，研究成果将为农作物的性状改良提供理论基础。

Light is one the most important environmental signals that regulating plant growth and development. Histone deacetylases widely participate in light-mediated gene expression and developmental processes, however, the mechanism keeps unclear. In our previous studies, we found that RPD3/HDA1-type histone deacetylase HDA15 interacts with bZIP-type transcription factor HY5, the key factor in light sigal transduction in Arabidopsis. HDA15 and HY5 co-regulate hypocotyl elongation and the expression of cell elongation related genes in Arabidopsis seedling. Furthermore, HDA15 affects the protein stability of HY5 in Arabidopsis. Based on these findings, in this study, we will analyze the genetic interplay of HDA15 and HY5 in regulating hypcotyl elongation. Next, we will study the target gene network and transcriptional mechanism co-regulated by HDA15 and HY5. Furthermore, we will also examine the mechinsim how HDA15 regulates the protein stability of HY5. These studies will elucidate the interplay of HDA15-HY5 module in regulating light sigal transduction, which may ultimately be applicable in improvement of properties of crops.