绿原酸（5-CQA）和3, 5-二咖啡酰奎宁酸（3, 5-diCQA）是杭白菊主要活性成分。超声波辅助提取具有低温、快速、高效、环保等优势，已应用于杭白菊深加工产业，然而其空化等效应可能导致该技术应用产生负面效应。本项目拟以杭白菊中的5-CQA和3, 5-diCQA为主要研究对象，借助HPLC-DAD、UPLC-QTOF-MS等技术手段，研究超声波空化效应、自由基效应等对咖啡酰奎宁酸异构化和降解的影响；通过建立不同咖啡酰奎宁酸降解动力学模型，确定咖啡酰奎宁酸结构与稳定性的关系；通过解析5-CQA和3, 5-diCQA的降解产物，揭示其在超声场下的降解途径和降解机理，为有效调控超声场下杭白菊功能成分的稳定性奠定理论和技术基础，以期为天然产物深加工产业提供新的理论依据与技术参考。

Chlorogenic acid (5-CQA) and 3,5-dicaffeoylquinic acid (3,5-diCQA) are two main bioactive ingredients of Chrysanthemum morifolium Ramat. Ultrasound assisted method has been applied in extraction of the bioactive ingredients from the plant because of its merits in light of its low extraction temperature, speed, high efficiency and environment-friendly technology. However, the cavitation effect of ultrasound may bring negative effects on the application of ultrasound extraction in Chrysanthemum morifolium Ramat. The objectives of this proposal include the investigation of the ultrasound extraction on stability of caffeoylquinic acids; and the influence of cavitation effect on isomerization and degradation of caffeoylquinic acids. In addition, degradation pathways and mechanisms of 5-CQA and 3,5-diCQA under the ultrasonic process will be investigated through analyzing structures of degradation products, which will be characterized by HPLC-DAD and UPLC-QTOF-MS. This project aims to reveal the negative effects of ultrasound assisted extraction on 5-CQA and 3,5-diCQA, as well as other bioactive ingredients, in Chrysanthemum morifolium Ramat, and provide scientific foundations in terms of the extraction optimization and deep understanding of the molecular stability of bioactive chemicals, for the industrial processing of the value-added medicinal plant.