病毒受体是抗病育种的重要靶标分子。基于前期工作基础，本项目将基于草鱼全基因组序列资料，针对II型GCRV代表性毒株GCRV-GD108，采用蛋白质组学分析方法，富集草鱼鳃、肠组织中的候选受体蛋白；通过体外蛋白质相互作用、细胞病毒结合阻断和体内基因编辑等技术手段，鉴定草鱼GCRV受体蛋白，评估其抗病育种潜力，为草鱼抗出血病品种培育提供关键靶标分子和技术支撑。

Virus receptors are important targets for molecular breeding of disease-resistant traits. Based on the basis of previous research and whole genome sequence of grass carp, according to grass carp reovirus strain GCRV-GD108 which is the representative strain of GCRV genotype II, this project will enrich some candidate receptor proteins in gill and intestine in grass carp using proteomic analysis. Then we will identify GCRV receptor proteins, and to evaluate their breeding potential for disease resistance by in vitro protein-protein interaction, virus blocking binding and in vivo gene editing techniques. This project will provide key target molecules and technical support for the cultivation of superior varieties of hemorrhage-resistant grass carp.