Characterisation of scavenger receptor class B type 1 in rare minnow (Gobiocypris rarus)
Characterisation of scavenger receptor class B type 1 in rare minnow (Gobiocypris rarus)
复制标题
稀有鲦鱼 (Gobiocypris rarus) 1 类清道夫受体的特征
DOI：
10.1016/j.fsi.2019.04.031
复制
发表时间：
2019-06
影响因子：
4.7
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Oi M;Huang R;Luo Q;Xiong L;Chen K;Wang Y
Scavenger receptor class B type 1 (SRB1) is a transmembrane protein belonging to the scavenger receptors (SRs) family and it plays an important role in viral entry. Not much is known on SRB1 in teleost fish. Grass carp reovirus (GCRV) cause huge economic losses in grass carp industry. In this study, rare minnow (Gobiocypris rarus) was used as a model fish to investigate the mechanism of GCRV infection, which is sensitive to GCRV. The structure ofSRB1gene inG. rarus(GrSRB1) was cloned and elucidated.GrSRB1is composed of 13 exons and 12 introns, and its full-length cDNA is 2296 bp in length, with 1521 bp open reading frame (ORF) that encodes a 506 amino acid protein. TheGrSRB1 protein is predicted to contain a typical CD36 domain and two transmembrane regions. InG. rarus,GrSRB1is expressed strongly in the liver (L), intestines (I), brain (B) and muscle (M), while it is expressed poorly in the heart (H), middle kidney (MK), head kidney (HK) and gills (G). After infection with GCRV,GrSRB1expression was up-regulated in main immune tissues during the early infection period. Moreover, co-immunoprecipitation assays revealed thatGrSRB1 could interact with the outer capsid protein of GCRV (VP5 and VP7). These results suggest thatGrSRB1 could be a receptor for GCRV. We have managed to characterize theGrSRB1gene and provide evidence for its potential functions for GCRV entry into host cells.
登录
查看更多内容
影响因子：
3.7
作者：
Langlois AC;Marinach C;Manzoni G;Silvie O
通讯作者：
Silvie O
影响因子：
30.3
作者：
Yalaoui, Samir;Huby, Thierry;Froissard, Patrick
通讯作者：
Froissard, Patrick
影响因子：
2.9
作者：
Ding Z;Luo N;Kong Y;Li J;Zhang Y;Cao F;Ye J
通讯作者：
Ye J
影响因子：
4.8
作者：
RIGOTTI, A;ACTON, SL;KRIEGER, M
通讯作者：
KRIEGER, M
清道夫受体 B 通过增强吞噬作用和调节抗菌肽的表达来保护虾免受细菌侵害。
影响因子：
2.9
作者：
Bi, Wen-Jie;Li, Dian-Xiang;Wang, Jin-Xing
通讯作者：
Wang, Jin-Xing