南方水果主要过敏原蛋白分子鉴定和分离纯化

近年来水果过敏问题呈现上升趋势，南方水果如荔枝、芒果、菠萝、以及亚热带新兴的杨梅水果在临床上都有严重过敏病例报道。受到公众、医学、果树学等科技界的关注。迫切需要在科学上提供致敏蛋白信息和相关诊断产品。国外开展了一些菠萝、香蕉、荔枝等热带水果的过敏原蛋白免疫学分析，但多数缺乏蛋白组分信息。在我国在这方面极少深入研究，对过敏原蛋白缺乏一个系统完整的鉴定分析，以至于不能进行可靠的水果过敏性评估和诊断预防。近年来相关果树基因组学、果实转录组学的进展为蛋白分子鉴定提供良好基础。本研究采用果实转录组、蛋白组和免疫学相结合的方法，对我国南方常见的过敏性水果（芒果、荔枝、菠萝和杨梅）进行主要过敏原蛋白的分子鉴定，分离纯化这些蛋白并进行理化性质和免疫学特性分析，分析不同水果间过敏交叉反应的蛋白分子基础，杨梅在欧洲的潜在过敏性评价。研究这些过敏原编码基因，并进行重组表达，应用于精准医疗的组分诊断。

Tropical and subtropical fruit allergy has been increased dramatically in recent years in China, it becomes a new food safety issue in social and scientific circles. Clinical fruit allergy of severe reactions had been reported in popular tropical fruits such as mango, pineapple, litchi, and also in new emerging subtropical fruit Chinese bayberry. There have been some reports on allergen identification by western countries, but very few in China, which hampers the accurate allergenicity assessment, proper diagnosis and prevention. Recent technology advancement in RNA-seq, proteomics and genome sequence project provided new approaches and solid bases to quickly identify allergens. This proposal will identify main allergen molecules in common southern China fruits (mango, pineapple, litchi and Chinese bayberry) by employing combined transcriptome, proteomic and immunological approaches. Purified natural and recombinant proteins will be investigated on their physical, chemical and immunological properties and the cross reactivity between different fruits at molecular level. Chinese bayberry is exotic fruit to European consumers, its allerenicity will be assessed by bioinformatics and serological tests. The expected results from this proposal will provide a comprehensive view of allergenic proteins of fruits in south China, and can further be explored in allergen component diagnosis.