含细胞的骨软骨复合支架综合3D成形机理及可控制造与试验研究

含细胞的骨软骨一体化复合支架设备的关键技术是生物制造的研究重点和难点，是人类健康迫切需要解决的民生问题，具有深远的研究价值和广泛的应用前景。本项目正是瞄准该关键技术领域,以含细胞的骨软骨一体化复合支架为对象，研究其制备机理和方法,综合多组分材料挤出、细胞电喷射等成形技术的优点, 运用流变学理论和CFD方法及数值模拟方法，建立成形过程中多物理场的分布及耦合关系, 构建非牛顿流体流动的行为模型；采用实验方法研究高压静电场对细胞生物活性的影响规律，将漏电介质、细长体模型与Maxwell本构方程结合构建细胞电喷的运动方程，模拟电场作用下纤维拉伸过程及含细胞的电纺纤维的附着形态；研究多尺度可控制造及调控机理模型、控制策略。利用研究成果建立细胞/支架综合成形试验平台,并进行大量试验研究,验证研究理论和技术的正确性,为研究直接制备含细胞的骨软骨一体化复合支架的设备提供理论支持和试验数据。

Key technologies of equipment for fabricating integrated composite scaffolds of osteochondral containing cells are the research emphasis and difficulties of Bio-manufacturing and the people's livelihood issue what is the urgent need to address, have the profound research value and broad application prospect. Aimed at these key technology areas and taking integrated composite scaffolds of osteochondral containing cells as the research object, the project researches the mechanism and methods of preparation, combines advantages of some technologies such as the reaction forming technology of multi-component materials、cell electrospray and so on, uses fluid rheology and CFD method to analyze and build distribution and the coupling relationships of multi-physics, builds the model of the isothermal flow behavior of non-Newtonian fluid, analyzes the survival of cells under high voltage electric field using experimental methods. Referencing leaky dielectric model and slender body theory, the project constructs the equations of motion of the cell electrospray by using Maxwell constitutive equation to simulate the drawing process of nano-fiber and researches the deposition of electrospinning containing cells to research the method of multi-scale controllable manufacturing and control mechanism model, control strategy. A large number of experiments will be done after establishing the cell/scaffold integrated forming platform to prove the correctness of the theories and technologies. This research project can provide theoretical support and testing data for the preparation of integrated composite scaffolds of osteochondral.