胞内DNA识别受体对逆转录病毒生活史中产生的DNA形式的识别为逆转录病毒的研究开启了新思路。但HTLV-1感染过程中哪种胞内DNA识别受体参与了病毒的识别和复制调控，目前尚不清楚。我们研究发现胞内DNA识别受体Ku70在PMA-THP-1细胞(PMA诱导分化的THP-1细胞，类似人的巨噬细胞)中由HTLV-1的感染诱导表达，且过表达Ku70抑制了HTLV-1病毒蛋白Tax和p19在mRNA水平上的表达。我们拟通过RNAi、westernblot、实时定量PCR、ELISA及荧光素酶报告基因实验等方法分别在人类原代单核细胞和PMA-THP-1细胞中探讨Ku70在HTLV-1感染过程中的功能及具体机制，并揭示Ⅲ型IFN在这一过程中扮演的角色。提出Ku70识别HTLV-1产生的DNA形式并负性调控HTLV-1复制的新观点，为深入理解HTLV-1感染相关疾病的病理过程提供了新角度。

Cytosolic DNA sensors are able to recognize the DNA forms produced during the life cycle of retroviruses and privide new angels about the research on retroviruses. However,there is little report about which cytosolic DNA sensor is responsible for HTLV-1 recognition and replication regulation. Our data showed that the expression of Ku70, which is a known cytosolic DNA sensor, was induced by HTLV-1 infection in PMA-THP-1 cells (PMA-differentiated THP-1 cells, a human macrophage-like cell line),and the overexpression of Ku70 inhibited the expression of HTLV-1 protein Tax and p19 at mRNA levels. We will investigate the role of Ku70 in HTLV-1 infection by the methods of RNAi silence, westernblot, realtime PCR, ELISA and luciferase assay etc. in primary human monocyte-derived macrophages (hMDMs) and in PMA-THP-1 cells. Furthermore, we will illuminate the role of IFN Ⅲ in this course. We would propose a new opinion that cytosolic DNA sensor Ku70 could recognize the DNA forms produced by HTLV-1 and negatively regulate the HTLV-1 replication. This research will provide new angles to understand the HTLV-1 infection associated diseases.