木尔坦棉花曲叶病毒（CLCuMuV）是引起棉花毁灭性病害棉花曲叶病的病原之一，由烟粉虱以持久循回方式传播。该病毒已入侵我国，并定殖和扩散。本团队发现，AsiaⅡ7烟粉虱传 CLCuMuV，而MEAM1烟粉虱不传，其分子机理不明。本项目将以CLCuMuV分离物GD01、AsiaⅡ7和 MEAM1烟粉虱为研究对象，应用亲和层析-质谱分析、膜体系酵母双杂交技术，筛选出AsiaⅡ7烟粉虱与CLCuMuV外壳蛋白互作的蛋白；利用BiFC、免疫共沉淀、GST pull down和基因沉默技术，鉴定出AsiaⅡ7烟粉虱传CLCuMuV的特异受体蛋白；分析该蛋白基因在AsiaⅡ7烟粉虱传毒循回中的表达，检测CLCuMuV与受体互作，进而解析AsiaⅡ7和MEAM1烟粉虱传播CLCuMuV能力差异的分子机理，为探索防控该病毒病新途径提供科学依据。因此，本项目具有十分重要的科学价值和应用前景。

Cotton leaf curl Multan virus (CLCuMuV) is one of pathogen causing the fatal cotton leaf curl disease in the world. The disease is transmitted by the whitefly, Bemisia tabaci in a persistent and circulative manner. The virus has invaded, colonized and spread in China. The applicant team has found that whitefly Asia Ⅱ7 cryptic species could transmit CLCuMuV, but MEAM1 cryptic species could not. Its molecule mechanism is unclear. In the study, we will use CLCuMuV isolate GD01, Asia Ⅱ7 and MEAM1 as materials, to screen the protein of Asia Ⅱ 7 interacting with CLCuMuV coat protein by affinity chromatograph, mass spectrometry, DUAL membrane yeast two hybrid technology, and to identify the specific protein of Asia Ⅱ7 whitefly with bimolecular fluorescence complementation (BiFC), Co- Immunoprecipitation, GST pull down test and RNAi technology. We also will analyze the gene express dynamic of the specific protein during the CLCuMuV circulation in Asia Ⅱ7 whitefly, and detect the interaction between CLCuMuV and the protein of Asia Ⅱ7 by immunofluorescence method. And then it would be revealed that the molecule mechanism of ability difference transmitting CLCuMuV between whitefly Asia Ⅱ7 cryptic species and MEAM1 cryptic species. It will provide the scientific basis for exploring the new way to control CLCuMuV. Therefore, the study has extremely important scientific value and application prospect.