Comparative transcriptome profiling reveals a network of differentially expressed genes in Asia II 7 and MEAM1 whitefly cryptic species in response to early infection of Cotton leaf curl Multan virus.
Comparative transcriptome profiling reveals a network of differentially expressed genes in Asia II 7 and MEAM1 whitefly cryptic species in response to early infection of Cotton leaf curl Multan virus.
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比较转录组分析揭示了 Asia II 7 和 MEAM1 烟粉虱神秘物种响应棉卷叶木尔坦病毒早期感染的差异表达基因网络
DOI：
10.3389/fmicb.2022.1004513
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发表时间：
2022
影响因子：
5.2
通讯作者：
He, Zifu
中科院分区：
文献类型：
--
作者：
Farooq, Tahir;Lin, Qi;She, Xiaoman;Chen, Ting;Tang, Yafei;He, Zifu
关键词：
木尔坦棉花曲叶病毒（CLCuMuV）是一种以白粉虱为媒介的菜豆花叶病毒，对世界范围内的几种重要经济作物构成严重威胁。CLCuMuV通过烟粉虱的差异传播主要依赖于烟粉虱隐种的类型。然而，不同的粉虱隐种之间的分子反应，以响应早期CLCuMuV感染仍然难以捉摸。在这里，我们比较了亚洲II 7和MEAM 1的隐蔽物种感染CLCuMuV的早期转录谱。Illumina测序结果显示，在CLCuMuV获得6和12 h后，与含病毒（VF）粉虱相比，含病毒（VF）亚洲II 7粉虱中有153和141个基因差异表达，而VF MEAM 1粉虱中有445和347个基因差异表达。亚洲II 7和MEAM 1中最丰富的差异表达基因（DEG）组分别与HTH-1和zf-C2 H2类转录因子（TF）相关。值得注意的是，与亚洲II 7相反，MEAM 1隐蔽物种显示出更高的转录变异，CLCuMuV感染后免疫相关反应升高。在这两个神秘的物种，我们确定了几个高度反应的候选DEG与抗病毒的先天免疫（α葡萄糖苷酶、LSM 14样蛋白B和磷酸烯醇丙酮酸羧激酶）、溶酶体（GPI锚定蛋白58）和自噬/吞噬体途径（隔离体-1，组织蛋白酶F样蛋白酶），剪接体（热休克蛋白70），解毒（细胞色素P450 4C 1），cGMP-PKG信号通路（肌球蛋白重链）、碳水化合物代谢（α-葡萄糖苷酶）、生物转运（线粒体磷酸盐载体）以及蛋白质吸收和消化（角质层蛋白8）。RNA-seq结果的进一步验证表明，28个选择的基因中有23个在RT-qPCR和RNA-seq中表现出一致的表达。我们的研究结果提供了重要的机制的见解，以了解不同的粉虱隐蔽物种的差异性Begomovirus传播的动态，并揭示了新的分子靶标昆虫传播的植物病毒的可持续管理。
Cotton leaf curl Multan virus (CLCuMuV) is a whitefly-vectored begomovirus that poses ramping threat to several economically important crops worldwide. The differential transmission of CLCuMuV by its vector Bemisia tabaci mainly relies on the type of whitefly cryptic species. However, the molecular responses among different whitefly cryptic species in response to early CLCuMuV infection remain elusive. Here, we compared early-stage transcriptomic profiles of Asia II 7 and MEAM1 cryptic species infected by CLCuMuV. Results of Illumina sequencing revealed that after 6 and 12 h of CLCuMuV acquisition, 153 and 141 genes among viruliferous (VF) Asia II 7, while 445 and 347 genes among VF MEAM 1 whiteflies were differentially expressed compared with aviruliferous (AVF) whiteflies. The most abundant groups of differentially expressed genes (DEGs) among Asia II 7 and MEAM1 were associated with HTH-1 and zf-C2H2 classes of transcription factors (TFs), respectively. Notably, in contrast to Asia II 7, MEAM1 cryptic species displayed higher transcriptional variations with elevated immune-related responses following CLCuMuV infection. Among both cryptic species, we identified several highly responsive candidate DEGs associated with antiviral innate immunity (alpha glucosidase, LSM14-like protein B and phosphoenolpyruvate carboxykinase), lysosome (GPI-anchored protein 58) and autophagy/phagosome pathways (sequestosome-1, cathepsin F-like protease), spliceosome (heat shock protein 70), detoxification (cytochrome P450 4C1), cGMP-PKG signaling pathway (myosin heavy chain), carbohydrate metabolism (alpha-glucosidase), biological transport (mitochondrial phosphate carrier) and protein absorption and digestion (cuticle protein 8). Further validation of RNA-seq results showed that 23 of 28 selected genes exhibited concordant expression both in RT-qPCR and RNA-seq. Our findings provide vital mechanistic insights into begomovirus-whitefly interactions to understand the dynamics of differential begomovirus transmission by different whitefly cryptic species and reveal novel molecular targets for sustainable management of insect-transmitted plant viruses.
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DOI：
10.1098/rstb.2018.0312
发表时间：
2019-03-04
影响因子：
6.3
作者：
Huo, Yan;Yu, Yuanling;Fang, Rongxiang
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Cilia, M.;Tamborindeguy, C.;Gray, S.
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影响因子：
6.7
作者：
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Zhang L
影响因子：
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Chang J;Block TM;Guo JT
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Guo JT
影响因子：
3.7
作者：
Gamage, Shirani M. K. Widana;Rotenberg, Dorith;Dietzgen, Ralf G.
通讯作者：
Dietzgen, Ralf G.