在轮奸案中获得的检材为2人及数人男性的精液混合物，Y-STR的检测可能做出排出的结论。单个精子因其为单倍体，不能提供全部遗传信息。本研究针对2人混合精液样品，联合检测Y、X、常染色体STR及用SSCP技术分离检测mtDNA高变区。可能分别检测出高、低拷贝者的Y、X、mtDNA高变区及部分常染色体STR型别，达到同一认定目的；无拷贝量差时，通过比对可排除或锁定嫌疑人。针对2人或多人混合精液样品，①捕获单个精子，检测mtDNA高变区序列，集合同型的精子进行常染色体STR分型。②应用荧光标记的抗A、B及H抗体，以免疫荧光组化技术捕获同型精子，进行常染色体STR分型。③根据精子表面多态性抗原的个体差别（ABO、HLA及筛选其他抗原），探讨应用流式细胞技术分离不同个体精子的方法。最终为轮奸案的侦破提供可靠证据。

Y-STR detection may make excluded conclusion when the samples obtained in the gang rape are two or several male semen mixture. As a haploid, the single sperm can't provide all the genetic information. In two mixed semen, this study can detect STR loci of Y chromosome, X chromosome and autosomes together and mtDNA hypervariable region after separation by SSCP techniques. If the content of the two samples is different, we can reach the same identified purpose by the detection of Y chromosomal loci, X chromosomal loci, mtDNA hypervariable region and parts of autosomal STR loci. The suspects can be ruled out or locked by comparison when the content is not significantly different. For the mixed semen of two or more samples: ①capture a single sperm, detect mtDNA hypervariable region, collect the same type of sperm and genotype autosomal STR loci. ②apply the fluorescent-labeled anti-A, anti-B and anti-H antibody to capture the same type of sperm by immunofluorescence staining technology, genotype autosomal STR loci. ③According to the individual differences of the polymorphic antigens of the sperm surface, explore the application of flow cytometry to separate the different individual sperm. Ultimately, our study can provide reliable evidence for the detection of gang rape cases.