混合斑的个人识别检验是法医物证鉴定领域中尚未很好解决的问题，特别是混合斑中低拷贝样品的分型检验，即使有嫌疑人经结果比对不能排除，但也不能肯定。本研究的基本设想是，应用新一代测序技术检测目前常用的STR试剂盒包含的常染色体与X染色体STR基因座其侧翼序列的SNP，筛选出侧翼序列SNP多态性好的足够数量的常染色体与X染色体STR基因座，根据个体间STR基因座侧翼序列SNP的不同，设计型特异性引物，用新一代测序技术或设计新的复合扩增体系，针对性的对混合样品中不同个体分别进行STR分型检测。以解决混合斑检验中的同一认定问题。

Personal identification of mixed stain in forensic evidence has not been well resolved, especially the typing test of low amount sample. Even if the suspects cannot be excluded, the comparison cannot make the confirmation. Our study is based on next-generation sequencing to detect the SNPs in the flanking region of the STR loci, which are included in the common autosomal and X chromosome STR detection kits. We select the sufficient polymorphic sites, which have linkage relations with the STR loci. Then, type-specific primers are designed to genotype the targeted STR sites of different individuals in mixed samples using next-generation sequencing or the new multiplex amplification system. To some extent, this method could resolve the identification in mixed stain.