已知肠源性内毒素血症(IETM)是导致酒精性肝损伤(ALI)的关键因素，而酒精引发的胃肠道功能紊乱、肠道通透性增加是形成IETM的始动原因。现有研究证实，乌药对急性ALI具有良好的保护作用，但具体作用机制不清。前期研究结果显示，乌药能降低血清炎症因子和门静脉LPS水平，改善小肠病理学改变，下调肝脏TLR4等的表达；结合乌药调节胃肠道功能、抗菌等作用，推断乌药可能通过调节肠道功能，保护肠粘膜屏障，减轻IETM，阻断LPS-TLR4介导的炎症级联反应而保护酒精性肝损伤。拟采用ELISA、RT-PCR及Western blot等方法，明确乌药对肠道功能、肠黏膜屏障及ALI的保护作用，阐述乌药调节肠道功能和保护ALI作用的相关性，阐明其抗ALI作用机制；通过体外Caco-2、RAW264.7细胞模型，观察乌药对紧密连接蛋白表达以及TLR4介导的炎症通路的影响，阐明其改善肠道通透性及抗炎作用机制。

As we know, intestinal endotoxemia (IETM) was the key pathogenic factor of alcoholic liver injury (ALI) and gastrointestinal disorder, intestinal flora maladjustment and high intestinal permeability induced by alcohol was the onset of IETM. As results of previous research show, Lindera aggregata (Wuyao in Chinese name, WY) has good protective effect on ALI, but the mechanism of WY on anti ALI was unrevealed. Previous researchs show that WY could siginificantly reduced the level of inflammatory factors in serum and LPS in portal vein, improve pathological changes of small intestine, downregulate the expressions of TLR4 et al.,. According to the effects of WY on regulating gastrointestinal function and anti-microbial, we deduced that WY could protect liver from alcoholic injury by regulating gastrointestinal function, protect intestinal mucosal barrier, allevialte IETM and inhabit inflammatory reaction induced by LPS-TLR4 signal pathway. As stated above, methods such as Elisa, 16S rRNA gene sequencing, RT-PCR and Western-blot and rats with alcololic liver injury were carried out to observe the effects of WY on anti alcoholic liver injury and to illuminate the relationship between gastrointestinal function regulating and ALI protection. Caco-2 and RAW264.7 cell models were used to observe the influence of WY on expression of tight junction associated protein and activation of inflammatory signal pathway mediated by TLR4, and to reveal the mechanism of WY on improving intestinal permeability and anti inflammation.