雌性大鼠出生前大气细颗粒物及其主要毒性组分苯并[a]芘暴露对育龄期生殖功能的远期效应及表观遗传学机制

胎儿是大气细颗粒物（PM2.5）的易受伤害群体，其生殖系统是PM2.5不良效应的敏感系统，目前罕有出生前PM2.5及其毒性成分暴露对雌性生殖健康远期不良效应的研究报道。项目以制备的本地区PM2.5及其主要毒性组分苯并[a]芘（BaP）制作雌性大鼠PM2.5或BaP暴露模型，观察出生前不同水平PM2.5或BaP暴露对胎鼠生殖系统影响所产生的出生后育龄期生殖功能的远期效应，从表观遗传学角度探讨BaP暴露效应的作用机制，阐释出生前BaP暴露-生殖系统损伤-育龄期生殖功能变化的内在联系。该研究将阐明出生前PM2.5及其主要毒性成分暴露对雌性大鼠生殖系统发育的不良效应及其作用机制，从易感群体安全性的角度为大气污染治理的规划目标和力度、大气污染允许标准的再修订提供科学依据，并为妊娠期母婴保健和优生优育施政提供重要参考。

Fetus is regarded vulnerable to environmental exposure, and its reproductive system as one of the most susceptible systems to airborne fine particulate matter (PM2.5). There have been rare research reports about long-term effects of prenatal exposure to PM2.5 and its toxic components on female reproductive health. In the present project, local PM2.5 particles will be prepared with their major toxic components analyzed. The long-term effects at child bearing ages of prenatal exposure to PM2.5 or its major toxic component benzo[a]pyrene (BaP) on female fetal reproductive system will be experimentally investigated in a murine model exposed to locally derived PM2.5 or BaP. The epigenetic mechanisms by which prenatal BaP exposure on female fetal reproductive system will be explored to uncover the intrinsic relations between BaP exposure and fetal reproductive injury/functional changes at child-bearing ages. By providing the experimental and mechanistic evidences for the long-term effects of PM2.5 and its major component BaP on fetal reproductive system, the present work may offer important evidences for understanding the negative impacts of PM2.5 and its toxic component on the development of female reproductive system and its underlying mechanisms. With regard to the safety of vulnerable subpopulations, this work will provide, more importantly, some critical evidences and references for future revision of air quality criteria for our national and local governments, an objective and necessity of social and economic needs for the management of air pollution. It is also essential to birth defect monitoring and maternal and infantile care.