睡眠是生物体的重要生理活动。睡眠失调与许多精神性疾病及神经变性疾病密切相关。对于睡眠觉醒调控以及睡眠障碍的研究，目前开展的工作主要集中于神经元。然而，胶质细胞作为脑内占绝大部分的细胞，其在睡眠觉醒调控以及睡眠障碍中的作用却仍不清楚。故我们制作了GFAP-ChR2-EYFP转基因大鼠，在星形胶质细胞上特异性地表达光敏感通道（ChR2）。本项目将综合运用光遗传学，结合光纤记录系统、在体多电极记录系统、新型腺苷探针实时检测等多种先进手段，探讨：1）面神经旁核的星形胶质细胞在睡眠觉醒中的作用及主动激活对睡眠觉醒的调节作用与模式；2）星形胶质细胞调节睡眠觉醒活动的化学物质机制；3）星形胶质细胞调节睡眠觉醒的神经微环路与突触机制；4）其是否有脑区差异性调节。本项目将揭示星形胶质细胞调控睡眠觉醒的作用及机制，提供理解睡眠觉醒调控的崭新方向与视角，可能为临床上睡眠障碍相关疾病的治疗提供新的参考。

Sleep is an important physiological activity of an organism. Sleep disorders are closely related to many mental and neurodegenerative diseases. The current work for the study of sleep-wake regulation and sleep disorders is mainly focused on neurons. However, glial cells, which are the majority of cells in the brain, their roles in sleep-wake regulation and sleep disorders are still unclear. So, our lab made GFAP-ChR2-EYFP transgenic rats, with ChR2 expressed specifically on astrocytes, so 473 nm blue light can specifically excite astrocytes, and sleep-wakefulness activities was recorded at the same time. This project will use optogenetics, combined with optical fiber recording system, in vivo multi-electrode recording system, adenosine probe real-time detection technique, to clarify: 1) the exact effect of astrocytes on sleep-wake regulation and the pattern of regulation; 2) the chemical mechanism of the effect of astrocytes on sleep-wake regulation; 3) the micro-circuits and synaptic mechanism of the effect of astrocytes on sleep-wake regulation; 4) whether there is a brain region difference of the effect. Our research will reveal the role and mechanism of astrocytic regulation of sleep-wake, and provide a new direction and perspective to understand sleep-wake regulation, which may provide a new reference for the treatment of clinical sleep disorders related diseases.