睡眠与记忆均是人类的重要生理活动。一定时间的睡眠能促进记忆的巩固。然而，由于促睡眠技术的限制，我们仍旧不太清楚如何睡眠才能促进记忆及其相关的机制。故本项目拟采用光遗传学技术在小鼠时间精确地诱导慢波睡眠（SWS），探讨：1）SWS对学习记忆的提高作用；2）SWS对学习记忆的作用时间模式；3）睡眠对学习记忆中的信息编码、巩固、提取的不同作用；4）利用动物研究的优势，采用病毒跨单级突触追踪、在体多电极记录神经元电活动与海马ripple、光遗传学刺激轴突末梢等方法，研究睡眠影响学习记忆的神经与环路机制。本项目将揭示精确调控睡眠对记忆不同阶段的作用，描绘海马的全脑传入投射图谱，阐明睡眠中海马-皮层环路的“crosstalk”机制。预期结果将为我们科学睡眠、提高记忆能力提供参考。

Sleep and memory are two important activities in human beings. We all know that a good sleep benefits memory. However, in the past, due to the lack of an effective technique that may specifically interfere sleep, the regulation models, the neural circuits and the mechanism of how sleep promotes memory are are still not clear. Recently, our preliminary study using optogenetic technique in VGAT-ChR2-EYFP transgenic mice showed that selectively excitation of GABAergic neurons in parafacial zone will generate and extend slow wave sleep (SWS). Therefore, in the present project we will generate SWS in mice to investigate: 1)The promoting effect of SWS on memory. 2)The temperal modes of the effect. 3) Different effects of SWS on memory encoding, consolidation and retrival. 4) The hipcampus-cortex neural circuits crosstalk and their mechanism using optogenetic axon terminal stimulation, in vivo multi-electrode recording of neural activity and hipcampal ripple oscillation, monosynaptic virus tracing and other means. This study will help to understand how sleep enhances memory. We will try to revel the underlying mechanism by drawing a map about the monosynaptic afferent projection of hippcampus and investigating the memory-related functional circuit connection during sleep.