“治疗性血管新生”被认为是心肌缺血后挽救缺血心肌和恢复心功能最具前景的补充治疗策略，周细胞在其中发挥关键作用，Ang-1/Tie-2旁分泌环路是调控周细胞功能和血管新生的重要通路。申请人前期研究发现黄芪-丹参成分药对具有明显的抗心肌缺血效应，还能调节周细胞-内皮细胞的Ang-1、Tie-2表达。据此提出黄芪-丹参成分药对可调控Ang-1/Tie-2旁分泌环路，进而调节周细胞功能和周细胞-内皮细胞相互作用，促进微血管新生并发挥抗心肌缺血效应的研究假设。本项目在中医药配伍理论指导下，以心肌缺血后血管新生为切入点，以周细胞为研究对象，通过采用条件性基因敲除小鼠和体外沉默Ang-1相结合的方法，探析黄芪-丹参成分药对调控Ang-1/Tie-2旁分泌环路、调节周细胞功能、促进血管新生以抗心肌缺血的作用机制和靶点，以期阐释中药成分抗心肌缺血的科学内涵，为黄芪丹参药对在心肌缺血后的临床应用提供科学支持。

"Therapeutic angiogenesis" is considered to be the most promising supplementary therapy strategy for myocardial ischemia, in which pericytes play a key role. Ang-1/Tie-2 paracrine loop is an important pathway to regulate pericyte function and angiogenesis. The previous study of the applicant found that Radix Astragali-Salvia Miltiorrhiza had obvious anti-myocardial ischemia effect and could regulate the expression of Ang-1 and Tie-2 in pericytes-endothelial cells. On the basis of this, this study puts forward the research hypothesis that Radix Astragali-Salvia miltiorrhiza can regulate Ang-1/Tie-2 paracrine loop, then regulate pericyte function and pericyte-endothelial cell interaction, promote microangiogenesis and exert anti-myocardial ischemia effect. Under the guidance of the compatibility theory of traditional Chinese medicine, taking angiogenesis after myocardial ischemia as the breakthrough point and pericytes as the research object, through the combination of conditional gene knockout mice and in vitro silencing of Ang-1, this study will explore the mechanism and target of Radix Astragali-Salvia Miltiorrhiza components on regulating Ang-1/Tie-2 paracrine loop, regulating pericyte function, promoting angiogenesis and anti-myocardial ischemia. The aim of this study is to excavate the scientific connotation of anti-myocardial ischemia of traditional Chinese medicine and provide scientific support for the clinical application of Radix Astragali-Salvia miltiorrhiza on myocardial ischemia.