水蛭Hirudo nipponica为药材水蛭的基原动物之一，是治疗心血管疾病中成药的主要原料，水蛭具有长期体内储藏食物的生存策略，目前有关水蛭食物储藏机理鲜见报道。本课题首先拟通过研究水蛭嗉囊及其储存食物在不同摄食阶段的差异及变化规律，寻找差异生物活性物质，并筛选明确嗉囊内主要共生微生物；其次分离纯化水蛭嗉囊及食物中具有抑菌功效的蛋白，测定其氨基酸序列并明确分类；根据分离纯化获得的蛋白并结合已有转录组数据，并克隆抑菌功能相关基因。综合对比分析筛选具有抑菌活性蛋白，并进行功能研究；最后分别通过测定相关抑菌基因的时空表达差异，阐明其作用途径，构建不同的平衡模式，从而揭示上述抑菌蛋白嗉囊内的作用机制，明确水蛭嗉囊长期储藏这一生存策略的机理研究，同时可为动物贮食行为研究提供新的理论基础。

Hirudo nipponica is the main raw animal of medicinal, which is one of the main raw meterials for a variety of proprietary Chinese medicine treatment of cardiovascular disease. The leech has long-term in vivo storage of food survival strategies. At present, the relevant reports are leech food storage mechanism. This paper intends to study the leech and crop food storage at different feeding stages and changes in the law, to find the difference of bioactive substances and screening of clear crop main symbiotic microorganisms; followed by separation and purification with antibacterial function of leech crop and food in the protein, determination of its amino acid sequence and clearly isolated according to classification; protein and combining existing transcriptome data, and cloning of genes related to antibacterial function. Comprehensive comparison and analysis of the food intake before and after difference material, purified antibacterial protein gene and molecular cloning of the corresponding protein, screening three mutually matched protein with antibacterial protein, and study the function; finally through space-time determination of antibacterial gene expression differences, clarify its mechanism, constructing different balance model thus, revealing the action mechanism of antimicrobial protein in the crop and the mechanism of long-term storage of leech crop. Meanwhile, the study can provide a new theoretical basis for the research on animal food storage behavior.