甜瓜枯萎病抗性基因Fom-2作用的分子机制研究

枯萎病（Fusarium wilt）是一种重要的世界性甜瓜病害，危害逐年加重，目前防治甜瓜枯萎病尚无突破性进展。解决甜瓜枯萎病最有效的途径是培育优良的抗病品种，抗病基因及抗病机制的研究对于抗病种质材料创新至关重要。因此，探索已克隆抗病基因作用的分子机理，了解抗病信号传导途径，发现信号途径中的关键基因将为甜瓜抗枯萎病研究开辟一个新的领域。 本研究在已克隆到Fom-2基因并验证了其抗病功能的基础上，拟进一步利用酵母双杂交技术、实时荧光定量PCR技术和遗传转化等手段，筛选调控Fom-2基因的转录因子及调节基因，查明与Fom-2基因表达及甜瓜枯萎病抗性相关的信号转导途径和代谢途径，揭示Fom-2基因的作用机制，同时获得新的抗性资源，为甜瓜抗枯萎病新基因的挖掘及种质创新奠定理论及材料基础。

Fusarium wilt is an important melon diseases in the world, causes important yield losses in melon crops worldwide. The most effective way is excellent resistant varieties. Resistance genes and the study of the mechanism of resistance are essential to the disease-resistant germplasm innovation. Therefore, the molecular mechanism of resistance genes have been cloned, signal transduction pathways and key genes in signaling pathways will opens up a new field of resistance. On the basis of cloned Fom-2 gene and verified the function of disease resistance, intends to further use of the yeast two-hybrid technology, means of real-time quantitative PCR technology and genetic transformation, to screen regulation Fom-2 gene transcription factor and regulated genes, to identify Fom-2 gene expression and melon Fusarium wilt signal transduction pathways and metabolic pathways, to reveal the role of the Fom-2 gene mechanism, obtain new resistance resources, lay the theoretical and material basis for melon anti wilt new gene screening and germplasm enhancement.