乙型脑炎病毒(Japanese encephalitis virus，JEV)感染引起人的神经系统疾病及猪的繁殖障碍，严重影响人类健康和养猪业的发展。对宿主自身防御和抵抗JEV感染的分子机制研究十分欠缺。我们的前期研究发现miR-124能够抑制JEV增殖，且其靶基因DNM2对JEV增殖具有促进作用，推测miR-124-DNM2基因-clathrin胞吞通路在抵抗病毒侵入细胞过程中发挥重要作用。本研究拟通过生物信息学预测和双荧光素酶报告基因系统鉴定miR-124介导的clathrin胞吞通路的靶基因，利用透射电镜观察并统计miR-124对JEV感染后clathrin胞吞小窝结构和数量的影响，最后验证靶基因在抗JEV感染中所行使的调节作用，从而阐明宿主miR-124通过调控clathrin介导胞吞通路抗病毒感染机制，为进一步将miR-124作为乙脑病毒抗病毒制剂的研发和猪抗病育种提供理论依据。

Japanese encephalitis virus (JEV) causes damage to the central nervous system in human and reproductive disorders in pig, therefore, JEV leads to seriously harm to human health and pork industry. Little is known about the molecular mechanisms of host defense and resistance to JEV. Our previous study found that miR-124 inhibited the proliferation of JEV and its target gene DNM2 facilitated JEV proliferation, then we speculated that miR-124-DNM2 gene-clathrin pathway played an important role in the resistance to JEV infection. In this study, the miR-124 target genes related to clathrin-mediated endocytosis pathway will be identified through bioinformatics prediction and dual-luciferase reporter gene system, then the structure and number of clathrin coated pits will be calculated using transmission electron microscopy in miR-124 transfected cells, the roles of target genes in JEV proliferation will be also studied. The results will reveal the molecular mechanism of viral inhibition by miR-124. It can also provide a theoretical basis to design agents against JEV and pig disease-resistant breeding.