Identification of Differentially Expressed Genes in Porcine Ovaries at Proestrus and Estrus Stages Using RNA-Seq Technique.
Identification of Differentially Expressed Genes in Porcine Ovaries at Proestrus and Estrus Stages Using RNA-Seq Technique.
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利用RNA-Seq技术鉴定猪发情前期和发情期卵巢差异表达基因
DOI：
10.1155/2018/9150723
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发表时间：
2018
影响因子：
--
通讯作者：
Zhao A
中科院分区：
文献类型：
--
作者：
Yang S;Zhou X;Pei Y;Wang H;He K;Zhao A
发情是影响母猪繁殖力的重要因素，与排卵和卵巢激素分泌有关。为了更好地了解猪发情的分子机制，利用RNA测序技术分析了发情前期和发情期卵巢mRNA的表达模式。与发情前期组相比，共鉴定出2,167个差异表达基因(≤0.0 5，|log2 Ratio|≥1)，其中784个基因表达上调，1,383个基因表达下调。基因本体论(GO)丰富表明，这些DEGS主要参与细胞过程、单个生物过程、细胞和细胞部分以及结合和代谢过程。此外，途径分析表明，这些DEG在京都基因和基因组百科全书(KEGG)的33条途径中显著丰富，包括细胞黏附分子、ECM-受体相互作用和细胞因子-细胞因子受体相互作用。实时定量逆转录聚合酶链式反应(qRT-PCR)证实了10个DEG的差异表达。本研究中发现的许多新的候选基因对于理解母猪发情周期的分子机制将是有价值的。
Estrus is an important factor for the fecundity of sows, and it is involved in ovulation and hormone secretion in ovaries. To better understand the molecular mechanisms of porcine estrus, the expression patterns of ovarian mRNA at proestrus and estrus stages were analyzed using RNA sequencing technology. A total of 2,167 differentially expressed genes (DEGs) were identified (P ≤ 0.05, |log2 Ratio| ≥ 1), of which 784 were upregulated and 1,383 were downregulated in the estrus compared with the proestrus group. Gene Ontology (GO) enrichment indicated that these DEGs were mainly involved in the cellular process, single-organism process, cell and cell part, and binding and metabolic process. In addition, a pathway analysis showed that these DEGs were significantly enriched in 33 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including cell adhesion molecules, ECM-receptor interaction, and cytokine-cytokine receptor interaction. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) confirmed the differential expression of 10 selected DEGs. Many of the novel candidate genes identified in this study will be valuable for understanding the molecular mechanisms of the sow estrous cycle.
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