玉米赤霉烯酮（ZEN）是一种由镰刀菌产生的类雌激素性质的毒素，具有极强的毒性，严重危害人畜健康。微生物降解毒素具有很好的应用前景。目前关于微生物降解ZEN的机理研究还不充分，完整的降解途径及参与基因尚不清楚。Bacillus amyloliquefaciens ZDS-1是本课题组分离的能降解ZEN的菌株，已经明确了该菌株的降解特性、田间控毒效果，并构建了ZDS-1的突变体文库。本研究以该菌株为研究对象，通过底物利用和产物鉴定技术剖析ZEN降解途径；借助于DNA高通量测序平台获得全基因组序列；运用基因组学和分子生物学相结合的方法鉴定ZEN降解相关基因，从基因水平阐明细菌降解ZEN的途径；研究脱毒酶基因的酵母表达和酶学特性。本研究结果将能解析细菌降解ZEN的途径及分子机理，为ZEN解毒酶制剂和转基因作物的开发奠定基础，为ZEN污染的生物控制提供理论和技术支撑。

Zearalenone (ZEN) is one of the estrogenic mycotoxins produced by Fusarium sp., which is very common in corns. ZEN is highly toxic and does great harm to the health of animal and humans. Degradation of ZEN by microorganism is the practical way to control ZEN contamination. However, the degradation mechanism of ZEN has not been completely elucidated, the complete degradation pathway and related genes still need to be studied. Bacillus amyloliquefaciens ZDS-1 was isolated in our lab; the strain can degrade ZEN completely. We have investigated the degradation characteristics of ZEN by strain ZDS-1 and its field application effects. We have constructed mutant library of Bacillus amyloliquefaciens ZDS-1 by transposon mutation. The target of this program is to elucidate the complete degrading pathway of ZEN by the method of substrates utilization and metabolites identification; to get the genomic sequence with the aid of new DNA sequencing platform, to clone the ZEN degrading genes involved in the pathway by the method of genome analysis combined with general molecular manipulation, and to express the ZEN detoxification genes in yeast and investigate the enzyme characteristics.. The result of this study will elucidate the degrading pathway and molecular mechanism of ZEN by ZDS-1, lay the foundation for the development of ZEN detoxification enzymes and genetically modified crops, and provide the theoretical and technical foundation for controlling ZEN contamination.