芥蓝种子萌发过程中芥子油苷代谢的microRNA调控机理研究

芥蓝种子萌发过程中芥子油苷含量，特别是脂肪类芥子油苷含量的下降使得芥蓝芽菜的品质受到极大影响，因此探讨芥蓝种子萌发过程中芥子油苷的代谢机制就显得尤为重要。最新研究表明microRNA（简称miRNA）可以通过调节芥子油苷的含量进而影响到植物在胁迫反应中的表现，然而miRNA如何调控次生代谢物芥子油苷的合成还未知。研究表明芥蓝在生长发育过程中芥子油苷含量呈现规律性的变化，特别是种子萌发过程中芥子油苷含量出现大幅度下降，是研究miRNA调控芥子油苷代谢的良好体系。本项目拟在芸薹属蔬菜芥蓝中考察miRNA对芥子油苷合成的调控作用，探明参与芥子油苷代谢调控过程中的miRNA以及芥子油苷合成过程中被miRNA调节的转录因子和合成基因，结合生物信息学分析和分子生物学技术阐明miRNA调控芥子油苷合成的分子机理。

The content of glucosinolate, especially aliphatic one, decrease in the germination of Chinese kale seeds, which cause the decline of the nutritional quality of Chinese kale sprouts. MicroRNA (miRNA) plays key roles in the development of plants, meanwhile, the accumulation of glucosinolate was changing accompanying with the growth and development of plant. Researchers have found that the change of expression levels of some miRNAs can alter the content of glucosinolate to cope with the stress caused by external environment. However, few information has been reported about how the biosynthesis of glucosinolate was regulated by miRNA. In this project we are going to identify miRNAs regulating the biosynthesis of glucosinolate in germinating Chinese kale sprouts and to clarify the mechanism underlying the regulation of miRNA on glucosinolate via seeking the transcriptional factors and biosynthetic genes of glucosinolate regulated by miRNA utilizing the bioinformatics and molecular biology.