乳腺癌严重威胁女性的健康和生命。目前，乳腺癌分子分型诊断主要依靠病理方法，但耗时长、创伤性大。利用影像技术，发展乳腺癌分子分型的体外快速检测方法对实现乳腺癌的精准诊疗意义重大。本项目拟构建基于上转换材料的磁/光响应型纳米探针，通过偶联不同亚型乳腺癌细胞的特异性靶向配体，实现对包括ER、PR、HER2阳性以及三阴性在内的乳腺癌分子分型体外特异性检测。重点研究磁/光响应纳米探针材料的制备与上转换发光/磁共振成像性能调控，不同亚型乳腺癌靶向配体筛选以及特异性纳米探针体系的构建，应用同步辐射X射线荧光（XRF）成像技术阐明纳米探针与不同亚型乳腺癌细胞相互作用的分子机制，实现全血样品中乳腺癌分子分型的“高灵敏性”和“高特异性”快速检测。本项目的研究有望为临床提供一种乳腺癌分子分型体外检测新方法，对实现乳腺癌的精准诊疗、改善预后具有重要的科学意义和潜在的临床应用价值。

Breast cancer seriously threaten the health and life in women. At present, molecular classification of breast cancer was generally diagnosed by pathology analysis, which was time-consuming and damaged for body. Using imaging technique, it is very important of developing in vitro detection method of molecular classification, which is meaningful for the precision theranostics in breast cancer. This project will construct magnetic/fluorescent-responsive nanoprobes based on upconversion nanomaterials, conjugate the specific ligands of different sub-typed breast cancer cells, and achieve the in vitro specific detection of molecular classification in ER-positive, PR-positive, HER2-positive and triple-negative breast cancer cells. Firstly, magnetic/fluorescent nanoprobes are synthesized and the performance of upconversion luminescence (UCL)/magnetic resonance imaging (MRI) is modulated. Then the specific nanoprobes of different sub-typed breast cancer cells are prepared by coupling the targeting ligands. Using synchrotron radiation X-ray fluorescence (XRF) imaging technique, the molecular mechanism of interaction is illuminated between magnetic/fluorescent-responsive nanoprobes and different sub-typed breast cancer cells. Finally, the in vitro rapid-detection of different sub-typed breast cancer cells in blood samples is achieved with high-sensitivity and high-selectivity. We believe that this research will provide a new method for in vitro rapid-detection of molecular classification in breast cancer, which will be very valuable for precision theranostics and good prognosis of breast cancer.