基于近红外光响应纳米探针的光声(PA)成像和光热治疗(PTT)在肿瘤可视化诊疗中具有重要的应用前景。然而，纳米材料的“低光转换效率”以及高通量光辐照引起的组织“非特异性热损伤”缺陷对光响应纳米探针的应用带来严峻挑战。本项目拟利用黑二氧化钛(TiO2)和金纳米星优异的PA成像/PTT性能，构建PA/光热性能增强的黑TiO2@金星核壳结构光响应纳米探针，实现在低通量近红外光辐照下对三阴乳腺癌的靶向PA成像/PTT。重点研究黑TiO2与金星之间的界面相互作用对PA/光热协同增强的影响；揭示RE-1/CK3双肽偶联对降低正常细胞非特异性摄取、提高三阴乳腺癌细胞靶向摄取的作用机制；阐明纳米探针注射剂量、光辐照通量与PA成像/PTT性能之间的关系。本项目构建的具有高光转换效率的黑TiO2@金星复合光响应纳米探针，对实现在低通量近红外光辐照下三阴乳腺癌的安全、高效靶向PA成像/PTT具有重要的意义。

Photoacoustic (PA) imaging and photothermal therapy (PTT) based on near infrared (NIR) light-responsive nanoprobes have important application in visualizing theranostics of cancers. However, the disadvantages of low photo conversion efficiency of nanomaterials and non-specific heating damage induced by high-flux light irradiation restrict their application of NIR-responsive nanoprobes in biomedicine. In this project, using the excellent PA imaging and PTT of black TiO2 and Au nanostars, PA/photothermal improved NIR-responsive nanoprobes of black TiO2@Au nanostars with core@shell structure are constructed for targeted PA imaging/PTT of triple-negative breast cancer under the irradiation of low-flux NIR light. This project will mainly focus on three aspects: (1) Investigating the effect of interfacial interaction between black TiO2 and Au nanostars on the synergistic enhancement of PA/photothermal performance. (2) Exploring the mechanism of decreasing the non-specific uptake of normal cells and increasing the specific uptake of triple-negative breast cancer cells by conjugating RE-1/CK3 double peptides. (3) Illuminating the relationship between nanoprobe dose, light flux and PA imaging/PTT performance. The as-constructed NIR-responsive nanoprobes based on black TiO2@Au nanostars with high photo conversion efficiency will be important for the safe, high-efficient, and targeted PA imaging/PTT of triple-negative breast tumor under the irradiation of low-flux NIR light.