闭花授粉性状可帮助作物实现自交，在作物产量形成、授粉控制和花发育生物学等方面研究上具有重要意义。前期研究中，本课题组利用新发现的甘蓝型油菜单显性位点控制（BnC03CL）的闭花性状种质，创建了稳定的近等基因系，并将闭花位点定位在C03染色体上的一个83 kb区间内。本研究拟在已有工作基础上，精细定位闭花性状位点，图位克隆闭花授粉基因，并从形态学、生理学、分子生物学等角度，研究闭花授粉性状的形成、分子调控网络以及闭花性状相关基因功能。

Researches on plant cleistogamy are of significance in uncovering mysteries in crop yield trait formation, pollination control system and flower developmental biology. In our previous works, we found a new dominant cleistogamous locus BnC03CL positioned on C03 chromosome within an interval of 83 kb in size in Brassica napus that is an often cross-pollinated crop with chasmogamous flowers. Present works will be focused on researches on the morphological, physiological properties of the BnC03CL near isogenic lines, and fine mapping and cloning the cleistogamous gene to reveal the roles played by the cleistogamous gene in flower development process with aid of molecular experimental techniques.