梨小食心虫是世界性的果树害虫，当前主要用性信息素诱捕雄蛾进行监测，但雌蛾的飞行能力显著强于雄蛾，尤其孕卵雌蛾可长距离迁飞至其它果园为害，有必要发展基于寄主挥发物的雌蛾监测技术。普遍认为普通气味结合蛋白在昆虫对寄主挥发物的嗅觉识别中起着重要作用，但在昆虫体内，该类蛋白是否同时参与昆虫与寄主植物及昆虫两性间的嗅觉识别尚不清楚。申请人前期研究结果发现，梨小食心虫2个普通气味结合蛋白基因的上调表达时间与该虫交配和产卵的时间相吻合。为此，本项目拟在此基础上，进一步测定梨小食心虫普通气味结合蛋白与性信息素和寄主挥发物的结合能力，分析普通气味结合蛋白结合不同气味分子的模式和具体生理功能，探明普通气味结合蛋白在梨小食心虫嗅觉识别中的功能角色。研究结果不仅有助于明确昆虫普通气味结合蛋白的生理功能，丰富昆虫嗅觉感受机制，而且对于快速筛选调节梨小食心虫成虫行为的寄主挥发物，制定合理的综合治理策略有重要参考价值。

Oriental fruit moth, Grapholita molesta (Busck), is a key pest of stone fruit throughout the world. Monitoring of G. molesta relies predominantly on pheromone trapping of male moths. However, the flight performance of this species exhibits marked sexual differences. In the field, female G. molesta are capable of making inter-orchard flights. This can pose a serious threat to other orchards. Therefore, a strategy for monitoring female moths, based on host plant volatiles, is desirable. It is widely believed that General odorant binding proteins (GOBPs) play an important role in the host recognition of insects. However, it is unclear whether the GOBPs of insect involved in both intraspecific and interspecific chemical communication. Our preliminary findings show that the high transcript levels of GOBP1and GOBP2 in G. molesta were detected at the end of photophase and during scotophase. This time is correlated with the mating and oviposition of G. molesta. In present study, we will further measure the ligand-binding specificities of GmolGOBPs with pheromones and host plant volatiles and investigate the mode of the binding interaction and the physiological function of GmolGOBPs in the detection of the compounds. This study not only have a great significance in elucidating the physiological function of GOBPs in insect chemical communication, but also be important to provide guidance for screening of plant volatiles regulating adult G. molesta behavior and facilitating the design of novel control strategies against G. molesta.