Molecular and Functional Characterization of pheromone binding protein 1 from the Oriental Fruit Moth, Grapholita molesta (Busck).
Molecular and Functional Characterization of pheromone binding protein 1 from the Oriental Fruit Moth, Grapholita molesta (Busck).
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东方果蛾信息素结合蛋白 1 的分子和功能表征，梨小食心虫 (Busck)
DOI：
10.1038/s41598-018-20719-0
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发表时间：
2018-02-02
影响因子：
4.6
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Zhang G;Chen J;Yu H;Tian X;Wu J
信息素结合蛋白（PBP）被认为主要是结合和运输蛾类的性信息素。累积的研究表明，在蛾类中鉴定出三种PBPs。在梨小食心虫中，GmolPBP 2和GmolPBP 3的功能已被研究。然而，GmolPBP 1的功能仍不清楚。此外，苹果蠹蛾性信息素Codlemone可作为苹果蠹蛾的性信息素。性骚扰Inc. Pomonella，CpomPBP 1特异性结合Codlemone。CpomPBP 1与GmolPBP 1具有较高的同一性（70%），表明这两个PBP可能具有相似的3D结构，因此可以结合相似或相同的配体。在这项研究中，我们探讨了GmolPBP 1的分子和功能特性。GmolPBP 1具有鳞翅目昆虫气味结合蛋白的典型特征，与CpomPBP 1在遗传学上最接近。结合实验表明，GmolPBP 1与（Z）-8-十二烯醇、1-十二烷醇和Codlemone具有较强的结合能力。分子对接表明，GmolPBP 1对三种配体具有不同的配体识别机制。这些结果表明，GmolPBP 1是（Z）-8-十二碳烯醇和1-十二碳醇混合物的识别分子，可能是Codlemone的潜在转运蛋白，参与了棉铃虫性信息素反应的协同作用。骚扰科德莱蒙
Pheromone binding protein (PBP) is thought primarily to bind and transport the sex pheromone in moths. The accumulated studies suggest that three PBPs were identified in moth species. InGrapholita molesta, the functions of GmolPBP2 and GmolPBP3 have been previously studied. However, the function of GmolPBP1 is still unclear. Furthermore, theCydia pomonellasex pheromone Codlemone can act as a sex pheromone synergist ofG. molesta. InC. pomonella, CpomPBP1 specifically bind the Codlemone. CpomPBP1 displays high identity with GmolPBP1 (70%), indicating that the two PBPs may share a similar 3D structure thus can bind the similar or same ligands. In this study, we explored the molecular and functional characterization of GmolPBP1. GmolPBP1, bearing the typical characteristics of Lepidopteran odorant binding proteins, was closest phylogenetically to CpomPBP1. Binding studies demonstrated that GmolPBP1 exhibited strong binding affinities with (Z)-8-dodecenyl alcohol, 1-dodecanol and Codlemone. Molecular docking showed that GmolPBP1 has different ligand recognition mechanism for the three ligands. Our results suggest that GmolPBP1 functions as recognizer of (Z)-8-dodecenyl alcohol and 1-dodecanol of the female sex pheromone blend, and may be the potential transporter of Codlemone, which contributes to the synergism of the pheromone response ofG. molestaby Codlemone.
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DOI：
10.1046/j.1432-1327.2001.01927.x
发表时间：
2001-02-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Briand, L;Nespoulous, C;Pernollet, JC
通讯作者：
Pernollet, JC
影响因子：
3.7
作者：
Ahmed T;Zhang TT;Wang ZY;He KL;Bai SX
通讯作者：
Bai SX
影响因子：
2.6
作者：
Ban, L;Scaloni, A;Pelosi, P
通讯作者：
Pelosi, P
粉红螟、大螟（鳞翅目：夜蛾科）三种信息素结合蛋白的性别偏向表达和信息素结合亲和力表明不同的作用
影响因子：
2.2
作者：
Jin, Jun-Yan;Li, Zhao-Qun;Dong, Shuang-Lin
通讯作者：
Dong, Shuang-Lin
通过触角转录组分析鉴定东方果蛾 Grapholitamolesta 的假定嗅觉基因。
影响因子：
3.7
作者：
Li G;Du J;Li Y;Wu J
通讯作者：
Wu J