创伤性脑损害（TBI）是严重的公共健康问题。课题组前期发现，miRNA-21、RhoB在TBI后表达变化及PTEN磷酸化与脑细胞凋亡及血脑屏障通透性关系密切，但miRNA-21对创伤后神经元及脑血管内皮细胞的确切作用和机理尚未阐明。我们推测，颅脑创伤后，miRNA-21通过调控PTEN和RhoB表达，启动下游通路，共同影响神经和血管的损伤修复。为验证此假说，本项目分别从组织和细胞水平观察不同损伤程度下miRNA-21表达变化，进一步通过合成针对miRNA-21的pre序列及其成熟体反向互补序列，构建慢病毒载体，利用病毒转染调控miRNA-21水平，观察其对PTEN、RhoB及下游通路的影响，并探讨上述变化对神经元及脑血管内皮细胞结构功能的影响。本课题不仅观察miRNA-21调控创伤神经元功能修复，更聚焦于脑血管内皮细胞损伤修复，期望达到更好的协同效果，为创伤后神经功能修复寻找新的治疗策略。

Traumatic brain injury (TBI) is an important public health problem. We found that the expression of miRNA-21 and RhoB after TBI and the phosphorylation of PTEN were associated with neuron apoptosis and the permeability of the blood brain barrier. However, the impact of miRNA-21 on neurons and cerebral vascular endothelial cells has not been elucidated. We postulated that miRNA-21 plays an important role in neurovascular restoration after TBI via the regulation of PTEN and RhoB expression. To verify this hypothesis, we will observe the expression of miRNA-21 at the tissue and cellular levels. The pre-sequence and reverse complementary sequence of miRNA-21 were synthesized first, and then regulate miRNA-21 via Lentivirus transfection. This study is to evaluate the changes in the PTEN and RhoB pathways at different miRNA-21 levels, and the impact of the changes on the structure and function of neurons and cerebral vascular endothelial cells. The project will not only observe neuron restoration after injury via miRNA-21 regulation but also focus on the restoration of cerebral vascular endothelial cells, in order to achieve a better synergistic effect, thereby we can find new treatment strategies for neurological function restoration after TBI.