Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola.
Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola.
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DOI：
10.1038/s41438-021-00533-y
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发表时间：
2021-05-01
影响因子：
8.7
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Liu GT;Wang BB;Lecourieux D;Li MJ;Liu MB;Liu RQ;Shang BX;Yin X;Wang LJ;Lecourieux F;Xu Y
野生葡萄对霜霉病病原体葡萄霜霉病菌表现出很强的抗性，但相关机制的描述很少，特别是在感染的早期阶段。在这里，我们进行了比较蛋白质组学分析的葡萄藤叶片从抗性基因型V. davidii“柳八-8”（LB）和敏感的V. vinifera“黑比诺”（PN）接种后12小时。通过采用iTRAQ技术，共444和349差异表达蛋白（DEP），分别在LB和PN。这些DEPs中的大多数与光合作用、呼吸作用、细胞壁修饰、蛋白质代谢、胁迫和氧化还原稳态有关。与PN相比，LB表现出较少的与光合作用相关的蛋白质的下调和更多的与代谢相关的蛋白质的上调。至少一个子集的PR蛋白（PR10.2和PR10.3）在两种基因型接种后上调，而HSP（HSP 70.2和HSP 90.6）和细胞壁相关的XTH和BXL 1蛋白特异性上调LB和PN，分别。在不相容的相互作用中，ROS信号通过H2 O2的积累而明显，并且多个APX和GST蛋白被上调。这些DEP可能在葡萄藤对霜霉病的反应中起着至关重要的作用。我们的研究结果提供了新的见解分子事件与葡萄藤霜霉病抗性，这可能会被利用，以开发新的保护策略，对这种疾病。
Wild grapevines can show strong resistance to the downy mildew pathogen P. viticola, but the associated mechanisms are poorly described, especially at early stages of infection. Here, we performed comparative proteomic analyses of grapevine leaves from the resistant genotype V. davidii “LiuBa-8” (LB) and susceptible V. vinifera “Pinot Noir” (PN) 12 h after inoculation with P. viticola. By employing the iTRAQ technique, a total of 444 and 349 differentially expressed proteins (DEPs) were identified in LB and PN, respectively. The majority of these DEPs were related to photosynthesis, respiration, cell wall modification, protein metabolism, stress, and redox homeostasis. Compared with PN, LB showed fewer downregulated proteins associated with photosynthesis and more upregulated proteins associated with metabolism. At least a subset of PR proteins (PR10.2 and PR10.3) was upregulated upon inoculation in both genotypes, whereas HSP (HSP70.2 and HSP90.6) and cell wall-related XTH and BXL1 proteins were specifically upregulated in LB and PN, respectively. In the incompatible interaction, ROS signaling was evident by the accumulation of H2O2, and multiple APX and GST proteins were upregulated. These DEPs may play crucial roles in the grapevine response to downy mildew. Our results provide new insights into molecular events associated with downy mildew resistance in grapevine, which may be exploited to develop novel protection strategies against this disease.
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影响因子：
5.6
作者：
Dai L;Wang D;Xie X;Zhang C;Wang X;Xu Y;Wang Y;Zhang J
通讯作者：
Zhang J
葡萄叶片蛋白质组的特异性调整可区分葡萄抗病基因型和易感基因型
影响因子：
3.3
作者：
Figueiredo, Andreia;Martins, Joana;Coelho, Ana Varela
通讯作者：
Coelho, Ana Varela
影响因子：
5.3
作者：
Gong Q;Yang Z;Wang X;Butt HI;Chen E;He S;Zhang C;Zhang X;Li F
通讯作者：
Li F
影响因子：
6.9
作者：
Chivasa, S;Hamilton, JM;Slabas, AR
通讯作者：
Slabas, AR
影响因子：
7.4
作者：
Carmo-Silva, A. Elizabete;Salvucci, Michael E.
通讯作者：
Salvucci, Michael E.