白头翁素(ANE)延缓细胞衰老的机制探索

随着社会老龄化时代的到来，关于衰老的机制和抗衰老途径，以及相关抗衰老药品的研究刻不容缓。我们从一些自中草药的小分子化合物提取物中进行初步筛选，发现白头翁素可能具有潜在的抗衰老活性。白头翁素从传统中草药白头翁中提取，临床曾用于镇痛以及消炎。经过实验验证，我们已经确定了白头翁素能够促进细胞增殖，延缓细胞衰老。为了寻找其中的靶点，我们使用白头翁素的分子结构在两种不同的数据库中进行靶点预测，并发现了PARP1分子。实验表明白头翁素可以抑制PARP1的表达，而且因为共有的底物NAD+，PARP1与SIRT1相互作用影响细胞衰老。本课题将采用细胞实验以及动物实验深入研究白头翁素在细胞衰老中的作用，并探索白头翁素-PARP1-SIRT1之间的相互作用机制，研究白头翁素作为衰老细胞清除剂的可行性，这将探讨衰老和衰老相关疾病的防治对策，从而达到实现“积极老龄化”的目标等具有重要意义。

With the advent of the aging society, it is urgent to study the mechanism of aging, the anti-aging pathway, and the related anti-aging drugs. We preliminarily screened the extracts of some small molecular compounds from Chinese herbal medicine and found that anemonin（ANE）may have potential anti-aging activity. ANE is extracted from the traditional Chinese herbal medicine Pulsatilla chinensis. After pilot-experiment, we have determined that ANE can promote cell proliferation and delay cell senescence. In order to find the target, we used the molecular structure of ANE to predict the target in two different databases and found the PARP1 molecule. It is suggested that ANE could inhibit the expression of PARP1, and because of the common substrate NAD+, PARP1 interacts with SIRT1 to affect cell senescence. .We will adopt Cell experiments and animal experiments to study role of ANE in cellular aging, explore the interaction between ANE - PARP1 - SIRT1 mechanism, and probe the feasibility of ANE as senolytics. This is of great significance for exploring prevention and control countermeasures of aging and age-related diseases, so as to achieve the goal of "active aging", etc.