油菜裂角抗性是一个机械化收获急待改良，能提高最终产量的重要性状。利用不同遗传群体对裂角抗性进行遗传解析，并定位克隆抗裂基因，明确油菜裂角抗性形成的分子机理，探明育种亲本的裂角基因等位变异，可有效指导油菜裂角抗性分子遗传育种。本项目拟开展以下研究：对油菜种质资源和遗传群体开展裂角抗性关联分析和连锁分析，鉴定裂角抗性稳定QTLs和骨干优良育种亲本中的各裂角位点抗或易裂的遗传背景；发展次级群体近等基因系精细定位抗裂QTLs，结合转录组分析和基因功能预测，定位抗裂候选基因并进行转基因功能鉴定；对影响抗裂性状的关键基因进行调控机制剖析，明确油菜裂角抗性基因形成的分子基础；从细胞结构、组织构成、生理角度研究油菜每个抗裂基因形成的生物学基础；开发裂角抗性等位基因特异标记或制定特异的基因编辑策略，针对已知抗性QTLs背景差异的骨干育种亲本材料，遗传导入或编辑改造缺失的基因抗性，精细调节育种亲本的裂角抗性。

The pod shattering resistance of rapeseed is an important agronomic trait which is beneficial to mechanized harvesting and can improve yield. The genetic analysis of pod shattering resistant resources, mapping and cloning of resistant genes, and clarifying the molecular mechanism of resistant traits can effectively provide technical support and theoretical basis for molecular breeding in rapeseed. This project intends to carry out the following research: GWAS and linkage analysis for rapeseed germplasm resources and genetic populations of pod shattering resistance are to identify the genetic background of stable resistant QTLs in elite breeding lines. Near-isogenic lines will be developed to fine mapping resistant QTLs. The candidate genes will be identified by combining transcriptome analysis and gene function prediction. Candidate genes will be identified by transgenic method furtherly. The key genes affecting shattering resistance will be analyzed to clarify the molecular basis in rapeseed. The biological basis for the formation of resistance genes will be studied from the aspects of cell structure, tissue composition and physiology. Specific markers of resistance alleles will be developed to target the background of known resistance or shattering prone QTLs. The elite breeding parents will be regulated the pod shattering resistance by introduction of resistance genes or editing the specific genes.