Expression in A. thaliana and cellular localization reveal involvement of BjNRAMP1 in cadmium uptake.
Expression in A. thaliana and cellular localization reveal involvement of BjNRAMP1 in cadmium uptake.
复制标题
DOI：
10.3389/fpls.2023.1261518
复制
发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
虽然芥菜已被证明是一种潜在的超积累作物，但目前还不完全清楚镉（Cd）在植物中的积累。在这里，我们发现，BjNRAMP1（天然抗性相关巨噬细胞蛋白1）通过其在酵母和拟南芥中的表达在镉和锰（Mn）的积累中起着至关重要的作用。高浓度镉暴露可诱导BjNRAMP1的表达。BjNRAMP1在酵母中的异位表达导致更高的积累的镉和锰相比，载体控制。BjNARAMP 1定位于质膜，并在根、叶和花的维管系统中表达。BjNRAMP 1在A.结果表明，外源Cd胁迫导致转基因植株根和地上部Cd积累量增加，抑制了植株的正常生长。此外，根系吸收锰激活镉胁迫的增加。总之，我们的研究结果表明，BjNRAMP 1显着有助于吸收锰和镉的B。Juncea。
Although Brassica juncea has demonstrated potential as a hyperaccumulator crop, it was not entirely clear how cadmium (Cd) accumulates in plants. Here, we found that BjNRAMP1 (Natural Resistance-Associated Macrophage Protein 1) plays a crucial role in the accumulation of Cd and manganese (Mn) through its expression in yeast and Arabidopsis thaliana. The high concentration of Cd exposure could induce the expression of BjNRAMP1. The ectopic expression of BjNRAMP1 in yeast led to higher accumulation of Cd and Mn compared to the vector control. BjNARAMP1 was localized to the plasma membrane and expressed in the vascular system of roots, leaves, and flowers. The overexpression of BjNRAMP1 in A. thaliana resulted in an increased accumulation of Cd in both roots and shoots, which inhibited the normal growth of transgenic lines. Moreover, Mn uptake in roots was activated by the increase in Cd stress. Together, our results indicated that BjNRAMP1 significantly contributes to the uptake of Mn and Cd in B. juncea.
登录
查看更多内容
影响因子：
4.6
作者：
Tang L;Mao B;Li Y;Lv Q;Zhang L;Chen C;He H;Wang W;Zeng X;Shao Y;Pan Y;Hu Y;Peng Y;Fu X;Li H;Xia S;Zhao B
通讯作者：
Zhao B
影响因子：
30.8
作者：
Kang L;Qian L;Zheng M;Chen L;Chen H;Yang L;You L;Yang B;Yan M;Gu Y;Wang T;Schiessl SV;An H;Blischak P;Liu X;Lu H;Zhang D;Rao Y;Jia D;Zhou D;Xiao H;Wang Y;Xiong X;Mason AS;Chris Pires J;Snowdon RJ;Hua W;Liu Z
通讯作者：
Liu Z
影响因子：
7.3
作者：
Peng, Jia-Shi;Ding, Ge;Gong, Ji-Ming
通讯作者：
Gong, Ji-Ming
影响因子：
3.7
作者：
Zhang D;Du Y;He D;Zhou D;Wu J;Peng J;Liu L;Liu Z;Yan M
通讯作者：
Yan M
影响因子：
6.9
作者：
Takahashi R;Ishimaru Y;Senoura T;Shimo H;Ishikawa S;Arao T;Nakanishi H;Nishizawa NK
通讯作者：
Nishizawa NK