近年来发现精氨酸耗竭是多种疾病常见的伴发现象，也是发病机制的重要促发（调控）因素。IFN-gamma除对免疫细胞调控作用，能够诱导组织细胞发生精氨酸耗竭，但其机制并不清楚。本项目拟以奶牛乳腺上皮细胞为模型，采用转录组学、核蛋白质谱技术和生物信息学技术，解析IFN-gamma刺激下胞内精氨酸代谢可能的途径和信号通路，筛选IFN-gamma刺激下参与精氨酸代谢的关键酶和蛋白分子；通过基因过表达、RNA干扰/基因敲除术，确证信号通路中关键节点分子，明确调控关系，体外、体内揭示IFN-gamma诱导精氨酸代谢的分子机制，识别和鉴定可用于人工干预或临床治疗的重要分子靶标。该项目的完成不仅提出IFN-gamma对奶牛乳腺组织细胞精氨酸代谢调控理论，为奶牛乳房炎防治提供新思路；而且有助于深入理解精氨酸异常代谢相关病症的发生机理，丰富细胞因子对组织细胞的调控知识。

In recent years, it has been found that arginine depletion is a common phenomenon which associated with many diseases, and is also an important precipitating(regulatory)factor in pathogenesis. In addition to the regulation of immune cells, IFN-gamma can also induce the depletion of arginine in tissue cells, but its mechanism is still not clear. This project intends to use bovine mammary epithelial cells as a model, using transcriptomics, nuclear protein mass spectrometry and bioinformatics to analyze the possible pathway and signaling pathways of intracellular arginine metabolism and screen the key enzyme and protein molecules involved in arginine metabolism under IFN-gamma stimulation; using gene overexpression, RNA interference / gene knockout technique to verify the key nodes molecular and clarify the mutual regulatory interaction, to reveal the molecular mechanism of IFN-gamma induced arginine metabolism, recognition and identification of important molecular targets for manual intervention or treatment. The completion of this project not only comes up with the regulation theory of IFN-gamma to healthy cow breast tissue cells of arginine metabolism, provides new ideas for prevention of cow mastitis and milk quality improvement; but also lay the foundation for the thorough understanding of IFN-gamma induced by arginine metabolism disorders, enrich the knowledge of regulation of cytokines on tissue cells.