猪胸膜肺炎放线杆菌(APP)是世界性严重危害养猪业的呼吸道致病菌，目前尚无理想的交叉保护性商品疫苗。Apa是本课题组前期发现的该菌重要的致病因子，肺巨噬细胞（PAM）是该菌感染后的主要受损细胞，系统研究Apa在PAM感染受损过程中的作用及机制是疫苗和药物研究的重要基础。因此本项目以Apa为研究对象，利用课题组已构建的Apa缺失突变株，采用转录组测序技术、iTRAQ技术等，系统检测缺失株和野生株黏附PAM并致其凋亡过程中的参与基因和蛋白分子，比较其不同，筛选出与Apa密切相关的调节分子及信号通路，在细胞模型和实验动物体内一一验证，从而全面揭示Apa在细菌与细胞黏附互作、被吞噬后胞内存活、诱导细胞凋亡阶段的作用机制，为猪传染性胸膜肺炎疫苗与药物研究提供急需的理论基础和靶标，并推动其它呼吸道感染的深入研究。这一成果完全属于自主创新，拥有自主知识产权，对呼吸道感染的防控具有重要科学意义。

Actinobacillus pleuropneumoniae （APP） is an important swine respiratory pathogen causing great economic losses worldwide. At present there is no ideal cross protective vaccine. Apa found by our team is a key virulence factor of APP triggering infection. And pulmonary macrophages (PAM) are the main damaged cells after APP's infection, so it is urgent and necessary to study deeply on function and mechanism of Apa in infection stage of swine pulmonary macrophages by APP strains for improvement of vaccine and drugs. With Apa as the object, based on our successfully constructed APP mutant missing Apa,this project aims to research function and mechanism of Apa in adhesion and phagocytose process, to explore APP survival mechanism in PAM, and to analyse signaling pathways of Apa mediating APP strains to induce PAM apoptosis when PAMs were infected by APPs, and to comfirm these results in animal model at last, through qPCR, transcriptome sequencing technology, iTRAQ technology. Thus revealing of the Apa role in adhesion, phagocytose, intracellular survival and inducing apoptosis process between bacteria and cell must provide much-needed theoretical basis and new targets for vaccine and drug research of swine contagious pleuropneumonia, and must promote the further research of other respiratory infections. This result is completely belongs to independent research, with independent intellectual property rights. And it has important scientific significance to the prevention and control of respiratory tract infection.