易转移复发和EGFR-TKIs耐药是NSCLC治疗的难点。HGF/c-Met通路的异常激活与非小细胞肺癌的复发转移和耐药发生密切相关，EMT在此发挥了关键作用，是当前研究的热点。本项目组从广西民间特色中草药，蒟蒻薯科,裂果薯属植物裂果薯中首次分离获得的裂果薯皂苷1具有明显抗NSCLC细胞侵袭转移和逆转吉非替尼耐药的作用，并初步确定与其抑制HGF/c-Met途径及调控EMT有关，且毒性低，已获专利。本项目将围绕HGF/c-Met/MAPK/ERK、HGF/c-Met/PI3K/AKT/mTOR信号通路和以此调控EMT，分别从细胞水平、生物整体水平，阐明SSPHⅠ抗NSCLC侵袭转移、逆转NSCLC对EGFR-TKIs耐药的分子作用机制和可能的作用靶点。本研究不仅有助于解决目前NSCLC治疗的的难点问题，而且也有助于揭示其治疗非小细胞肺癌的作用提供实验理论依据。

It is difficult to treat non-smal cell lung cancer (NSCLC) with easy metastasis and relapse and EGFR-TKIs resistance. Abnormal activation of HGF/c-Met pathway is closely related to recurrence, metastasis and drug resistance of NSCLC. Epithelial-mesenchymal transition (EMT) plays a key role in this process. Saponins from Schizocapsa plantaginea (Hance) Brenth, Schizocapsa, Taccaceae as natural produts from Guangxi folk characteristic Chinese herbal medicine isolated and obtained by our project team can significantly inhibit invasion, metastasis and reversal of gefitinib resistance of NSCLC cells. It has been preliminarily determined that the saponinⅠ is related to inhibiting HGF/c-Met pathway and regulating EMT with low toxicity, and the patent authorization. Based on HGF/c-Met/MAPK/ERK, HGF/c-Met/PI3K/AKT/mTOR signaling pathway and regulation of EMT, this project will will be investigated that the molecular mechanisms and possible targets of SSPH I in anti- invasion and metastasis of NSCLC and reversing resistance of NSCLC to EGFR-TKIs at the cellular and biological levels respectively. This study is not only helpful to solve the current difficulties in the treatment of NSCLC, but also hopeful to reveal the mechanism of NSCLC treatment and provide experimental theoretical basis for the development of a new anti-NSCLC Chinese medicine based on modern medical and molecular biology knowledge.