新疆维医临床常用疏花蔷薇果治疗腹泻遗精，腰腿酸痛，咳嗽气喘，口舌生疮等症，前期研究发现其乙酸乙酯和正丁醇提取部位具有明确的体外降脂、抗肿瘤活性，其中乙酸乙酯部位含芦丁、山奈酚、金丝桃苷等黄酮类成分含量达62%，但其调脂药效成分及作用机制未见报道。 .本研究以柱层析、制备液相、MS、NMR等分离鉴定技术结合体外肝脂肪变性模型筛选、追踪疏花蔷薇果调节NAFLD脂质代谢的活性成分；采用Caco-2细胞模型研究活性成分的膜吸收特性，为有效成分确定提供药动学依据；并在整体动物水平评价调脂有效部位的安全性和药效作用；结合“血清药物化学”和“血清药理学”方法，研究调脂有效部位含药血清对脂联素、AMPK、SREBP-1c、ACC、PPARα等与脂质合成、转化相关的各代谢信号通路基因表达的影响，从分子水平研究其多部位、多靶点的综合调节脂质代谢的机理，为开发防治NAFLD的口服制剂提供科学依据。

Rosa Laxa R. fruits is commonly used as a traditional Uygur medicine in xinjiang for treatment of diarrhea, spermatorrhea, waist and leg aches, cough and asthma, aphthae boil of the tongue. Previous research has shown that its ethyl acetate extraction and n-butanol extraction have obvious regulating blood lipids and antitumor activity in vitro. Meanwhile，the flavonoid content of the ethyl acetate extraction，including rutin, kaempferol，hyperin and so on，was 62%. At present，the effective constituents of regulating blood lipids of Rosa Laxa R. fruits and its action mechanism are still unclear. . Based on previous research results，some separation and detection technology，including column chromatography，preparative HPLC，MS、NMR and so on，combined two hepatic steatosis cell models，will be applied to directed screening the active constituents of regulating lipids metabolism from Rosa Laxa R. fruits. The film absorption characteristics of active components were researched by Caco-2 cell model, which will provide the pharmacokinetic basis of effective constituents. To evaluate the safety and pharmacodynamics of effective parts in rats. Based on the methods of serum pharmacochemistry and serum pharmacology，the mechanism of regulating lipids metabolism will be systematically investigated based on the multi-site, multi-targe on the molecular level. To study the related gene expression influence of serum containing the active constituents by the main metabolism signal pathways of lipid synthesis, transformation，such as Adiponectin, AMPK, SREBP-1c, ACC, PPARα in hepatic steatosis cells. It will provide experimental basis for the research and development of preventing and treating for NAFLD oral drug from Rosa Laxa R. fruits in the future.