乙型肝炎病毒（HBV）Dane's 颗粒组装机制一直是传染病病原学研究领域的未能解决的问题之一。导致病毒特异性靶向药物研发滞后，HBV 感染难以根治。本研究通过：（1）对HBV 复制相关人类功能未知的糖基因aer61 的克隆表达及初步功能研究，通过对aer61 基因编码的AER61 蛋白在HBV 包膜蛋白糖基化修饰内质网质控过程中的作用，以期阐述AER61 影响HBV 亚病毒颗粒组装和分泌过程中的作用。（2）重点探讨了AER61 蛋白与内质物甘露糖苷酶I 可能的相互作用，通过构建aer61 高表达和基因敲除模型，比较分析aer61 表达变化对HepG2.2.15 细胞亚病毒颗粒表达及相应修饰N-聚糖糖型的影响，以期阐述HBV 包膜蛋白修饰N-聚糖糖型变化与亚病毒颗粒分泌之间的关系。本研究有助于HBV 复制分子机制的诠释。

The particles assembling of Hepatitis virus B is a main problem in area of hepatitis pathogensis, which is still unresolved at present. This situation is results in antiviral therapy without virus eradicated effects. The present study is designed, (1) to clone and characteristic of a new glycogene, aer61, and elucidate the role of aer61 in HBV particles assembling and secretion, through exploring the mechanism of aer61 mediated quality control with glycosylation. (2) to investigate association of aer61 and mannose glycosidase. The glycoform analysis was performed to compare glycan in HBV particles and subparticales, with cell model of aer61 overexpressed and gene-knock-down in vitro. The present study is aim at the relationship between the glycoform of N-glycan on surface protein and HBV particles secretion.