白念珠菌是临床极为常见的条件致病真菌，其致病机理备受关注。由形态发生信号转导网络及分泌途径决定的形态发生过程，是该病原菌得以有效侵染宿主及应答多种环境压力的重要原因。我们的前期研究表明，白念珠菌钙信号系统不仅对于细胞钙稳态维持及环境压力应答至关重要，而且与形态发生过程紧密相关。本项目旨在通过形态发生表型分析，明确各种钙信号成员在白念珠菌形态发生及侵染宿主细胞过程中的重要作用；通过形态发生相关基因GFP报告系统，探索钙信号成员在调节形态发生信号转导网络中的功能；采用顶体及肌动蛋白染色、形态发生相关蛋白定位等手段，探讨其对分泌途径的调节功能；通过细胞壁组分分析及RT-PCR技术，分析其在细胞壁完整性维持中的作用。本项目旨在全面揭示钙信号系统对形态发生信号转导网络及分泌途径的调节作用，明确其介导形态发生过程的分子机制，以丰富白念珠菌的形态发生理论，并为临床治疗白念珠菌感染提供新的思路。

Candida albicans is one of the most important opportunistic fungal pathogens, and its pathogenic mechanisms were being paid attention. Morphogenesis, which is governed by morphogenesis transduction network and the secretory pathway, is the critical factor for this pathogen to effectively invade and respond to various environmental cues. Recently, we found that the calcium signaling system is not only required for maintenance of cellular calcium homeostasis and stress response, but also is closely associated with morphogenesis in this pathogen. In this study, we will clarify the role of C. albicans calcium signaling members in morphogenesis and invasion to host cells through phenotype assays, and explore the functions of these members in regulating the morphogenesis transduction network. Moreover, we will investigate the important role of calcium signaling members in the secretory pathway by actin and Spitzenkörper staining, and explore their functions in maintaining cell wall integrity by cell wall composition assays and real-time PCR. This study aims to clarify the role of C. albicans calcium signaling system on morphogenesis transduction network and secretory pathway, enrich the fungal morphogenesis theory, and provide new strategies against C. albicans-related infections.