大豆茸毛色是重要的形态学标记，涉及大豆抗病虫等重要农艺性状。在很多作物中，色素沉积的分子调控机理已经比较清晰，但大豆中控制茸毛色形成的基因尚未被完全了解。前期我们定位并克隆了Td基因，发现Td基因编码一个R2R3型MYB转录因子，该转录因子主要通过调控黄酮的含量影响大豆茸毛色。本项目在此基础上利用编辑突变体验证Td基因的功能，筛选鉴定Td基因的互作蛋白与靶标基因，进而解析MYB转录因子调控茸毛色形成的分子机制。该项目的完成，将在一定程度上丰富大豆茸毛色在分子水平上的认知，为大豆分子育种提供基因资源及理论基础。

Pubescence color is an important morphological marker corresponding to diseases and insects in soybean. Although pigmentation has been well dissected at molecular level in different plant species, the genes controlling natural variation of pubescence color were not fully understood in soybean. We have recently located and cloned Td gene. Td gene encoded an R2R3 MYB transcription factor, which affects pubescence color by regulating the accumulation of flavonoids. Thus, in current project: Function of Td gene will be identified by gene editing; Interaction protein and target genes related with Td gene will be screened and identified. The molecular mechanism of MYB transcription factor regulating pubescence color formation will be disclosed. The completion of this project will fill the gap in understanding of Td gene at the molecular level and provide genetic resources and theoretical basis for soybean molecular breeding.