Combined Transcriptomic and Metabolomic Analysis Reveals the Role of Phenylpropanoid Biosynthesis Pathway in the Salt Tolerance Process of Sophora alopecuroides.
Combined Transcriptomic and Metabolomic Analysis Reveals the Role of Phenylpropanoid Biosynthesis Pathway in the Salt Tolerance Process of Sophora alopecuroides.
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DOI：
10.3390/ijms22052399
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发表时间：
2021-02-27
影响因子：
5.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Zhu Y;Wang Q;Wang Y;Xu Y;Li J;Zhao S;Wang D;Ma Z;Yan F;Liu Y
盐分胁迫是限制作物产量和农业发展的主要非生物胁迫。因此，研究盐胁迫对植物的影响以及植物对盐胁迫的响应机制势在必行。在本研究中，我们利用转录组学和代谢组学的方法来探讨盐胁迫对苦豆子的影响。我们发现盐胁迫在0、4、24、48和72h引起了显著的基因表达和代谢产物的变化。综合转录和代谢组学分析表明，在盐胁迫下苯丙烷生物合成途径中获得的差异表达基因(DGs)和差异代谢产物(Dms)显著相关。其中，28个DEGS和7个DM参与木质素的合成，23个DEGS和7个DM参与类黄酮的合成。盐胁迫下，与木质素和类黄酮合成相关的基因和代谢产物的表达发生了显著变化。木质素和类黄酮类物质可能参与了苦豆子根组织中ROS的清除，减轻了盐胁迫对其造成的伤害。本研究为研究植物对盐胁迫的响应机制，进一步提高植物的耐盐性提供了新的思路和遗传资源。
Salt stress is the main abiotic stress that limits crop yield and agricultural development. Therefore, it is imperative to study the effects of salt stress on plants and the mechanisms through which plants respond to salt stress. In this study, we used transcriptomics and metabolomics to explore the effects of salt stress on Sophora alopecuroides. We found that salt stress incurred significant gene expression and metabolite changes at 0, 4, 24, 48, and 72 h. The integrated transcriptomic and metabolomic analysis revealed that the differentially expressed genes (DEGs) and differential metabolites (DMs) obtained in the phenylpropanoid biosynthesis pathway were significantly correlated under salt stress. Of these, 28 DEGs and seven DMs were involved in lignin synthesis and 23 DEGs and seven DMs were involved in flavonoid synthesis. Under salt stress, the expression of genes and metabolites related to lignin and flavonoid synthesis changed significantly. Lignin and flavonoids may participate in the removal of reactive oxygen species (ROS) in the root tissue of S. alopecuroides and reduced the damage caused under salt stress. Our research provides new ideas and genetic resources to study the mechanism of plant responses to salt stress and further improve the salt tolerance of plants.
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影响因子：
2.3
作者：
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通讯作者：
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影响因子：
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