周期性牵张应力作用下OPG/RANKL/RANK信号通路调节牙骨质细胞分化响应机制的实验研究

牙根吸收的过程是几个分子途径和因素相互作用的结果，严重的牙根吸收会导致牙齿的松动脱落。牙骨质细胞在正畸治疗所致炎性牙根吸收的修复中发挥重要作用，既往研究表明牙骨质细胞的体内表达谱与骨细胞平行，但目前关于牙骨质细胞在牙根吸收修复过程中的作用及其临床应用的研究未见文献报道。本项目通过周期性牵张应力刺激小鼠牙骨质细胞系IDG-CM6，观察不同拉伸形变量对牙骨质细胞增殖活性及功能活性的影响，研究张应力刺激对牙骨质细胞OPG/RANKL基因表达的调控及ERK1/2与P38（信号蛋白）表达的变化，为OPG/RANKL/RANK信号通路对牙骨质细胞的成骨调控提供研究基础，为预防和治疗正畸治疗中牙根吸收修复提供实验依据。

The process of root resorption is the result of the interaction of several molecular pathways and factors. Serious root resorption can induce tooth loosening and exfoliation. Cementocyte play an important role in the repair of inflammatory root resorption induced by orthodontic treatment. Previous studies have shown that the expression profile of cementocyte in vivo is parallel to that of osteocytes. However, there are few studies on the clinical application value of cementocyte and their role in the process of root resorption and repair. In this project, cyclic strain is used to stimulate mouse cementocyte line IDG-CM6 to observe the effects of different stretch strain on the proliferation and functional activity of cementocyte. We study the regulation of the expression of OPG/RANKL gene and the changes of ERK1/2 and P38 (signal protein) in cementocyte, which provide the basis for the regulation of OPG/RANKL/RANK signaling pathway on osteogenesis of cementocyte and provide an experimental basis for the prevention and treatment of root resorption and repair in orthodontic treatment.