BAF复合物利用其ATP酶亚基水解ATP产生的能量改变组蛋白与DNA的作用，从而调控基因表达。BAF复合物在大脑的发育中起着关键的作用。找到连接BAF复合物特异因子调控组蛋白修饰同该因子所承担生物功能之间的特定靶基因一直是BAF复合物研究中的最大挑战。我们的研究显示在神经干细胞的分化过程中，BAF复合物因子Dpf2的缺失导致神经前体细胞的滞留，同时非成熟及成熟神经减少。我们的初步研究表明Dpf2可能通过调节增强子的H3K27ac修饰，调控神经干细胞中相关靶基因表达。因此，本课题将主要研究：1，利用体外分化系统和小鼠模型，研究Dpf2在体内，体外小鼠神经干细胞自我复制及神经发育中的功能；2，Dpf2在神经干细胞中与其功能相关的关键靶基因的筛选；3，Dpf2如何协同组蛋白特定修饰，神经干细胞的重要因子在靶基因上的结合，调控关键靶基因的表达；3，Dpf2在人的神经干细胞中的功能及其机制。

The BAF complex uses the energy produced by its ATPase subunit to hydrolyze ATP to alter the action of histones and DNA, thereby regulating gene expression. The BAF complex plays a key role in the development of the brain. It has been the biggest challenge in the BAF complex study to find specific target genes that link BAF complex-specific factors to regulate histone modifications with the biological functions of this factor. Our study shows that the loss of the BAF complex factor Dpf2 leads to the retention of neural precursor cells and the reduction of immature and mature nerves during differentiation of neural stem cells. Our preliminary study suggests that Dpf2 may regulate the expression of related target genes in neural stem cells by modulating the H3K27ac modification of the enhancer. Therefore, this subject will focus on: 1. Using in vitro differentiation systems and mouse models to study the function of Dpf2 in neural stem cell self-renewal and neural development; 2. Screening of key target genes for Dpf2 function in neural stem cells; 3, how Dpf2 synergizes with histone-specific modification, binding of important factors of neural stem cells to target genes, and regulates the expression of key target genes.