宫颈癌是最常见的妇科恶性肿瘤之一，严重威胁了女性的健康。宫颈癌化疗耐药是导致化疗失败及影响预后的重要因素，因此提高宫颈癌化疗药物的敏感性是提高宫颈癌治疗效果的关键。在我们的前期研究中，发现S100A9蛋白的表达与宫颈鳞癌的发生相关，同时发现化疗前宫颈鳞癌组织中S100A9蛋白的表达与顺铂为基础的新辅助化疗的敏感性相关，但具体机制尚未明确。近年的研究表明，宫颈癌细胞对顺铂的敏感性与细胞凋亡等多种因素相关。为此，本项目拟结合体内和体外试验，通过质粒转染、RNA干扰、流式细胞仪、抗体芯片等技术，深入研究S100A9对宫颈鳞癌细胞凋亡、增殖、迁移和侵袭等生物学行为的影响；研究S100A9对宫颈鳞癌细胞顺铂化疗药物敏感性的影响及其相关的关键蛋白和信号传导通路，阐明S100A9对宫颈鳞癌顺铂化疗敏感性影响及机制，为提高宫颈癌的化疗效果提供新的思路和实验依据。

The incidence rates of cervical cancer are highest in gynecological cancers, which threat women's health. Chemo-resistance of cervical cancer cells is an important factor for leading to chemotherapy failure and poor diagnosis. Therefore, to improve the sensitivity of cervical cancer to chemotherapy treatment plays an important role in improving the treatment effects on cervical cancer. In our previous study, we found the expression of S100A9 was associated with carcinogenesis of cervical squamous cancer. In addition, S100A9 protein expression in pre-chemotherapy cancer tissues could predict response to cisplatin-based neoadjuvant chemotherapy in cervical squamous cancer. However, the mechanism of them is still unclear. In recent years, studies reported that chemo-sensitivity of cervical cancer cells to cisplatin was related to many factors including cell apoptosis. In the present study, combined with in vivo and in vitro studies, using construction of recombinant plasmid, RNA interference, flow cytometry, and antibody array, we will explore the effects of S100A9 on malignant characteristic of cervical squamous cancer cells, such as cell apoptosis, cell proliferation, invasion and migration, and investigate the effects of S100A9 on chemo-sensitivity of cervical squamous cancer cells to cisplatin, as well as related role protein and signal transduction pathway. This study will reveal the effects and mechanism of S100A9 on chemo-sensitivity of cervical cancer cells to cisplatin and provide a new thread and experimental basis for improving the chemotherapy effects on cervical cancer.