乳酸菌解除呕吐毒素对肉鸡免疫毒性的机制研究

呕吐毒素广泛存在于受霉菌污染的谷类作物中，被动物采食后可诱发免疫抑制性疾病，表现为疫苗免疫效价降低，对病原体易感性增加。乳酸菌可以促进免疫器官发育，改变细胞因子分泌类型，提高机体自身免疫调节能力。本项目拟探讨通过在肉鸡日粮中添加乳酸菌增强机体免疫调节能力，以解除饲料呕吐毒素对肉鸡免疫抑制作用的可行性及相关机制，期望为饲料呕吐毒素污染造成肉鸡免疫力下降的问题找到有效的解决办法。首先通过观察乳酸菌对免疫细胞 IL-10与 IL-12等细胞因子分泌的变化关系，筛选出具有高效免疫调节功能乳酸菌；再经过离体试验探讨乳酸菌是否能改善因呕吐毒素诱导的IL-10与IL-12调节失衡及CD4+CD25+细胞功能异常；最后通过在体试验验证乳酸菌解除饲料呕吐毒素对肉鸡免疫抑制作用的效果。本项目的实施对于应用乳酸菌缓解呕吐毒素诱导肉鸡免疫抑制具有重要的理论价值，对于饲料安全及公众健康具有重要的现实意义。

Deoxynivalenol widely exists in cereal crops contaminated with mold. When it is fed to chickens, the mycotoxin may induce immune suppression, characterized by increasing susceptibility of pathogens and reducing vaccine titers. Probiotics may possess immune-enhancing activities and protect the host from infection by pathogens through strengthening the immune organ development and improving the secretion of cytokines. This project is designed to study the effects of Lactic acid bacteria on reducing immunosuppression induced by the mycotoxin in broiler chickens. First, lactic acid bacteria with efficient immune regulating function will be screened by measuring the changes of IL-10 and IL-12 in immune cells. Then, whether lactic acid bacteria can correct the imbalance of IL-10 and IL-12 and dysfunction of CD4+CD25+cells induced by the myxotoxin will be investigated in vitro. Finally, the effects of lactic acid bacteria on abrogating immune toxicity of deoxynivalenol in broiler chickens will be explored in vivo. It is expected that results from this proposed project will provide theoretical support for adopting lactic acid bacteria to on abrogating immune suppression of deoxynivalenol in broiler chickens and contribute to feed safety and public health.